Labeling index experiments with 3H-thymine deoxyriboside
Cells are cultured to the appropriate density, labeled with 1H -thymidine deoxyriboside for 30 min, washed and fixed, and unadulterated precursors are removed i
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Elimination of microbial contamination
Cultures were washed several times with high concentrations of antibiotics by rinsing monolayer cultures or by resuspending non-adherent cells by centrifugation
Cell viability assay by dye uptake assay
Living cells can take up diacetylfluorescein and hydrolyze it to fluorescein, which cannot pass through the cell membrane of living cells [Rotman and Papermaste
primary outgrowth
The tissues were minced and washed, and the tissue blocks were inoculated on the surface of culture flasks or Picot dishes with a small amount of medium contain
human biopsy tissue
In consultation with hospital personnel, provide labeled containers with culture media and arrange to have samples taken from the operating room or pathologist.
Oral keratinocytes
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
prostate epithelium
The prostate was removed and the specimen processed (30 min), and the prostate tissue was digested with collagenase (1 h) to collect individual cells and small
Proliferation assay of mouse pluripotent ES cells
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Clone formation assay of adherent cells
The cells were treated with different concentrations of experimental reagents for 24h. After trypsin digestion, the cells are inoculated at low cell density, in