Restriction fragment length polymorphism (RFLP) analysis
Restriction fragment length polymorphism (RFLP) is one of the most important analytical methods in molecular biology and is mainly used to (1) detect DNA sequen
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Sequencing reaction experiments
To get the right signal intensity, adjust the amount of DNA according to the size of the DNA fragments. For ABI PRISM Big Dye Terminator Ready Reaction mix (App
Precision amplification experiments for large fragments
Amplify a ~5kb fragment with 10 different primer pairs, the template DNA is the same, the reaction system is 50ul, and the control has no template and contains
PCR-SSCP technology experiment
Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technology can be used for: (1) screening and detection of oncogenes and oncogenic
PCR product purification assay
A method is described for the purification of PCR products using an AmiconMicrocon-PCR filter (Millipore) that efficiently removes redundant dNTP and primers. T
PCR product quantification
Accurate quantification of the template prior to sequencing is critical. Depending on the amount of DNA used, several different methods are available. Two other
Preparation of RNA by ultracentrifugation with guanidine isothiocyanate/cesium chloride
This method is derived from a modification of the protocol developed by Chirgwin et al. (1979). The reagents specified for Chirgwin et al. are commonly used in
An experimental method for the isolation of genomic DNA based on magnetic beads
The Wizard Magne Sil Genomic DNA Purification System uses a solid-phase paramagnetic silica pellet to purify genomic DNA, replacing vacuum filtration and centri
Genomic DNA isolation experiment based on silica membrane
Silica chemistry has become one of the methods for purifying high-quality genomic DNA from samples such as mouse tail clippings, animal tissues, and tissue cult