Reverse transcription synthesis of single-stranded cDNA experiment
The volume of a cDNA synthesis reaction is dependent on the number of anchored-anywhere primer combinations used. The protocol provided below, applies to 24 pri
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Amplification of 3'-end cDNA by classical RACE
This experiment describes the amplification of 3'-end cDNA by classical RACE.PCR Lab Guide, Section 25.4, Protocol 1
FDD-PCR experiment
This protocol is designed as 24 PCR reactions using 3 cDNA subpopulations (RT reactions utilizing the H-T11M primer from protocol 3) with primers that are a com
subtractive hybridization experiment
The experiment introduces subtractive hybridization, which is derived from PCR Laboratory Guide (Second Edition) by Seed Kang and Qu Lijia.
Re-amplification, cloning and sequencing experiments of differentially expressed cDNAs
After cutting the latent differentially expressed cDNA from the acrylamide gel, the cDNA is re-amplified with the same anchored-any primer combination under the
Differential hybridization experiments with detection and drive DNA probes
The following 4 probes are used for differential screening hybridization. 1. detector-specific ablation probe (forward ablation probe). 2. driver-specific ablat
Genomic DNA removal experiment from total RNA
For FDD gene expression analysis, as well as the use of any other RNA-based gene expression technique, contaminating genomic DNA must be removed prior to revers
Northern blot analysis assay
To confirm that the selected cDNAs are indeed differentially expressed, it is recommended that Northern blot analysis be used instead of other confirmatory tech
PCR-based DNA library screening experimental methods
This experiment introduces the PCR-based DNA library screening method. This experiment is derived from PCR Laboratory Guide (Second Edition) by Seed Kang and Qu