Molecular Markers - AFLP Principles and Procedures
AFLP can be used for (1) construction of genetic linkage maps; (2) rapid identification of molecular markers closely linked to target genes using AFLP; (3) AFLP
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Dual PCR experiment
With the large-scale cultivation of genetically modified (GM) crops, the safety of GM crops has received increasing attention, and in 2002, China required the l
Colony PCR experiment
Conventional PCR amplification requires multiple steps of bacterial culture and plasmid preparation before gene amplification, which is cumbersome and time-cons
PCR-single-stranded conformational polymorphisms
PCR-single-strand conformation polymorphism is a technique based on this DNA single-strand conformation property combined with gel electrophoresis to detect gen
Homologous one-step fluorescent allele-specific PCR
Homologous one-step fluorescent allele-specific PCR utilizes water-soluble conjugated polyelectrolytes (CPs) containing a large number of absorbing units, and t
Cold denaturation co-amplification PCR
Cold denaturing co-amplification PCR is a method for selectively amplifying a small number of alleles from a mixture of wild-type and mutant-containing sequence
single-cell PCR
Single-cell PCR utilizes flow cytometry to isolate a specific type of cell. Using a single cell, PCR can be analyzed at the DNA or mRNA level to find molecular
Quantitative PCR
Quantitative PCR is a technique to quantify the amount of PCR starting template by analyzing the PCR end product or monitoring the PCR process using a standard
Single-molecule PCR
Single-molecule PCR, also known as homo-primer PCR, is a PCR technique in which a single DNA molecule is used as a template and the number of cycles is unlimite