Cyclic nucleotide-dependent protein kinase analysis assay
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Experiments for direct analysis of kinases in gels
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Experimental determination of protein concentration by spotted filter membrane binding method
The spotted filter binding method is a rapid method for the initial determination of protein content. This method is particularly useful for screening useful fr
Experiments on the purification of proteins by filtration chromatography using filter gel
Gel filtration chromatography is an important protein purification technique, also known as size exclusion, gel exclusion, molecular sieve, or gel filtration ch
Experiments on the purification of proteins by ion exchange chromatography
Ion exchange chromatography is the most widely used of the chromatographic means for purifying proteins. It has a high resolution for proteins, is easy to perfo
Dye-matched base chromatography for protein purification experiments
Dye-ligand chromatography is not really affinity chromatography because they are not natural ligands for the proteins to which they bind. However dye columns bi
Immunopurification experiments
Antibody affinity purification is one of the most effective methods in protein purification. This step alone often results in 1000 to 10,000-fold purification.
Interaction cloning experiments
Interacting cloning (also known as expression cloning) is used to identify and clone proteins encoding proteins that interact with a target or 'bait' protein. T
Dissolution and reduction of samples for two-dimensional polyacrylamide gel electrophoresis
Source : Proteomics: From Sequence to Function.