Fura-2 AM Staining Protocol
This protocol has been successfully adapted to different cell types, including fibroblasts, PC12 cells, and embryonic neurons, with different Fura-2 AM concentrations corresponding to different cell densities .
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Immunopeptide blocking assay
Sometimes, antibodies may bind non-specifically to proteins other than the antigen. This is usually more common with polyclonal antibodies, but can also occur with monoclonal antibodies.
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
Enzyme -linked immunospot (ELISPOT) is a technique related to ELISA that is used to detect secreted proteins such as cytokines and growth factors .
In-cell ELISA protocol
In-cell ELISA (also known as cell-based ELISA, cell western blot, or cytoblot) is a widely accepted immunocytochemistry assay that uses a colorimetric or fluorescent readout to quantify a target protein or post-translational modifications of a target protein in cultured cells.
GST Pull-Down Protocol
In cells, proteins participate in extensive protein-protein interaction networks that appear as dynamic "protein machines" that assemble and disassemble in response to a constantly changing influx of intracellular, intercellular, and extracellular cues. An initial step in understanding protein ...
Immunohistochemistry (IHC) staining antigen retrieval protocol
Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...
RNA dot blot protocol
RNA dot blotting is a technique similar to Northern blotting; however, RNA samples cannot be separated by electrophoresis and require membranes for imaging .
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry staining with samples in paraffin (IHC-P)
Immunohistochemistry staining with samples in paraffin (IHC-P)