Laboratory Protocols & SOPs Library
- Experiments on oxidation and phosphorylation in isolated mitochondria SummaryWhen sufficient oxygen is supplied to plant tissues, plant cells can oxidize substrates completely. When glucose is fully oxidized as a respiratory substrate, CO2 is produced at the end, the absorbed O2 is reduced to water, and the oxidation of each gram molecule of glucose produces 38 ...
- Experiments on the substitution of hydrogen and copper for magnesium in chlorophyll SummaryChlorophyll molecule has a porphyrin ring, its magnesium ions are located in the center of the porphyrin ring, chlorophyll molecule is more unstable, in contact with acid, the central magnesium is easy to be replaced by hydrogen, in the presence of other heavy metal ions, hydrogen is also ...
- Experiments on the determination of photosynthetic intensity of plants SummaryAccording to the similarity of the structure and physiological function of the two sides of the leaf midvein, you can first cut off half of the leaf in the dark, the remaining half of the leaf to be carried out for a certain period of time after photosynthesis, there is an accumulation of ...
- Experiments on the compositional analysis of plant wounding fluids SummaryPlant root system is not only an important organ to absorb water and mineral elements, but also an important synthetic organ, plant absorbed inorganic salts, some of which is the primary assimilation in the root, transformed into organic matter and transported to the ground.When the above ...
- Simple identification experiments of plant dehydrogenases SummaryRespiration can be thought of as a series of oxidative and reductive actions, the intermediate steps of which are facilitated by a variety of oxidative and reductive enzymes, and the presence of respiratory enzymes is identified by the choice of special substrates or hydrogen acceptors ...
- Simple identification experiments of plant oxidizing enzymes SummaryOxidases dehydrogenate from metabolites and allow them to combine directly with oxygen to produce water or hydrogen peroxide. Examples include cytochrome oxidase, ascorbate oxidase, and polyphenol oxidase.Operation methodSimple identification experiments of plant oxidizing ...
- Experiments on the determination of water potential in plant tissues by the small fluid flow method SummaryThe water status of plant tissues can be expressed in terms of water potential. The magnitude of the water potential of plant tissues can, to a certain extent, reflect the water demand status of plants. Generally speaking, when the plant tissue water potential is low, it is a reflection ...
- Simple identification experiments of plant peroxidases SummaryCatalase is a hemoglobin-like enzyme that contains iron in its composition and catalyzes the decomposition of hydrogen peroxide into water and molecular oxygen. During this process, the iron atoms in the cofactor undergo alternating oxidizing and reducing changes.Operation methodSimple ...
- Experiments on the antagonistic effects of single-salt toxicity and mixed salts SummaryMonosalt toxicity and antagonism are related to hydrophilic colloids in protoplasm and protoplasmic membranes; the higher the ionic valence, the lower the concentration required to eliminate the toxic effects of the monosalt. Mineral ions, especially cations, have a tremendous influence ...