GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Probably involved in nervous system development and function.
숙주 종
마우스
특이성
FGF12
면역원
Recombinant protein of human FGF12 (AA 71-240)
포지티브 제어
WB: Rat brain, Mouse brain lysates. IP: Rat brain lysates.
변화
Unconjugated
등급
Carrier Free, ExactAb™, Validated
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A purified
집중력
1.0 mg/mL
보관 및 배송
모양
Liquid
보관 조건
Store at -80°C,Avoid repeated freezing and thawing
배송
Dry ice packs + Cold packs
안정성 및 스토리지
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
FGF12 Mouse mAb (Ab103281) - Western Blot All lanes: FGF12 Mouse mAb (Ab103281) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 22 kDa Exposure time: 157.3 s
FGF12 Mouse mAb (Ab103281) - IP Immunoprecipitation was performed by incubation of 2.5 µg of K94041_8C12 with rat brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. FGF12 Mouse mAb (Ab103281) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Rat brain lysate Lane 2: FGF12 immunoprecipitated from rat brain lysate by FGF12 Mouse mAb (Ab103281) Lane 3: The isotype control antibody instead of FGF12 Mouse mAb (Ab103281) in Rat brain lysate. Result: FGF12 Mouse mAb (Ab103281) can immunoprecipitate FGF12.
적용
적용
Dilution info
WB
1/1000
IP
1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
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Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FGF12 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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