GBP4 Antibody - 1차 항체, specific to GBP4, Rabbit IgG

Cat. No.: Ab104956

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

토끼

Reactive species

인간

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human GBP4 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:

WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
Flow Cytometry: 20 µg/mL

IF/ICC: 2.5 µg/mL

Protein Function:
Binds GTP, GDP and GMP. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Plays a role in erythroid differentiation. 

Specifications

Product Name

GBP4 Antibody - 1차 항체, specific to GBP4, Rabbit IgG

동의어

GBP 4antibody | GBP-4 antibody | GBP4 antibody | GBP4_HUMAN antibody | GTP binding protein 4 antibody | GTP-binding protein 4 antibody | Guanine nucleotide binding protein 4 antibody | Guanine nucleotide-binding protein 4 antibody | Guanylate binding prot

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

숙주 종

토끼

특이성

GBP4

면역원

Recombinant GBP4 expressed in E.coli (AA 1-292).

포지티브 제어

WB: A431 cell lysate. IHC: Human breast cancer tissue. Flow Cytometry: A431 cells. IF/ICC: HeLa cells.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified + Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: GBP4 Antibody (Ab104956)

GBP4 Antibody (Ab104956) - Western Blot
All lanes: GBP4 Antibody (Ab104956) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 72 kDa
Exposure time: 6.5 s

IHC: GBP4 Antibody (Ab104956)

GBP4 Antibody (Ab104956) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20 µg/mL GBP4 Antibody (Ab104956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Flow Cytometry: GBP4 Antibody (Ab104956)

GBP4 Antibody (Ab104956) - Flow Cytometry
Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100, then stained with 20 µg/mL GBP4 Antibody (Ab104956) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.

IF/ICC: GBP4 Antibody (Ab104956)

GBP4 Antibody (Ab104956) - IF/ICC
IF analysis of GBP4 Antibody (Ab104956) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GBP4 Antibody (Ab104956) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• Flow Cytometry:

  20μg/mL;

• IF/ICC:

  2.5 µg/mL ;

관련 대상(인간)

GBP6 Tbio Guanylate-binding protein 6 (0 Activities)

Discover Target: GBP6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

GBP4 Tbio Guanylate-binding protein 4 (0 Activities)

Discover Target: GBP4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)