Hsp27 마우스 mAb - 1차 항체, specific to Hsp27

Cat. No.: Ab210504

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG2b

Clone Number

CD04/1E2

Conjugation

Unconjugated

개요

마우스 항 인간 Hsp27 항체, 클론 CD04/1E2는 WB, 유세포 분석법, ELISA 등에 사용할 수 있습니다.

Specifications

Product Name

Hsp27 마우스 mAb - 1차 항체, specific to Hsp27

동의어

열충격 27kDa 단백질 항체 | 28kDa 열충격 단백질 항체 | CMT2F 항체 | DKFZp586P1322 항체 | 부고환 분비 단백질 Li 102 항체 | 에스트로겐 조절 24kDa 단백질 항체 | 에스트로겐 조절 24kDa 단백질 항체 | Hea

사양 및 순도

ExactAb™, 검증됨, 동물 무항생제, 보균자 무함유, 아지드 무함유, 고성능, ≥95%(SDS-PAGE), 1.0 mg/mL

생화학 및 생리적 메커니즘

변성된 단백질을 접을 수 있는 상태로 유지하는 분자 샤프론 역할을 하는 작은 열충격 단백질. 스트레스 저항과 액틴 조직에 중요한 역할을 함. 분자 샤프론 활동을 통해 세포의 수를 조절할 수 있음

숙주 종

마우스

특이성

Hsp27

면역원

Recombinant protein corresponding to Human Hsp27

포지티브 제어

WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells

변화

Unconjugated

등급

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

라이트 체인 유형

kappa

SDS-PAGE

150.0 KDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

보관 및 배송

보관 조건

20°C에서 보관하고, 냉동과 해동을 반복하지 마십시오

배송

아이스 박스 + 아이스 패드

안정성 및 스토리지

4°C에서 단기 보관(1~2주). 20°C 또는 -80°C에서 보관하세요. 냉동/해동 주기를 피하세요.

이미지

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 1.7 s 

Western Blot: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab210504) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 2.8 s 

Flow Cytometry: Hsp27 Mouse mAb (Ab210504)

Hsp27 Mouse mAb (Ab210504) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab210504) (Red) at 0.125 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).​

적용

• 적용: Dilution info
• WB:

  1/1000 - 1/20000 ;

• Flow Cytometry:

  1/1000 - 1/100,000

관련 대상(인간)

HSPB1 Tchem Heat shock protein beta-1 (0 Activities)

Discover Target: HSPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp27 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.