MED18 Antibody - 1차 항체, specific to MED18, Rabbit IgG

Cat. No.: Ab114765

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human MED18 Antibody, Polyclonal, could be used for WB and so on.

Application:

WB: 1/500-1/2000

Protein Function：

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Specifications

Product Name

MED18 Antibody - 1차 항체, specific to MED18, Rabbit IgG

동의어

SRB5 | p28b | FLJ20045 antibody | hMED 18 antibody | hMED18 antibody | Human Mediator of RNA Polymerase II Transcription antibody | MED 18 antibody | med18 antibody | MED18_HUMAN antibody | Mediator complex subunit 18 antibody | Mediator of RNA polymerase

사양 및 순도

ExactAb™, Validated, 1.0 mg/mL

숙주 종

토끼

특이성

MED18

면역원

A synthetic peptide derived from human MED18 (AA 70-119)

포지티브 제어

WB: Jurkat, A431, A549 and C6 cell lysates.

변화

Unconjugated

등급

ExactAb™, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Immunogen affinity purified

집중력

1.0 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: MED18 Antibody (Ab114765)

MED18 Antibody (Ab114765) - Western Blot
All lanes: MED18 Antibody (Ab114765) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 24 kDa
Observed band size: 22 kDa
Exposure time: 60.0 s

적용

• 적용: Dilution info
• WB:

  1/500-1/2000 ;

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MED18 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.