NMDI14 - 10mM in DMSO , CAS No.307519-88-6

CAS: 307519-88-6 Cat. No.: N655628 Molecular Weight: 415.51 PubChem CID: 2886617

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

NMDI14 is a nonsense mediated RNA decay ( NMD ) inhibitor. NMDI14 disrupts the SMG7-UPF1 interactions and inhibits NMD .

In Vitro

NMDI14 is a nonsense mediated RNA decay (NMD) inhibitor. Treating cells with NMDI14 for 6 hours leads to an increase of PTC 39 β globin to 12%, a relative four-fold increase that, if resulting in biologically active hemoglobin, would be sufficient to ameliorate the clinical symptoms of thalassemia. Three days of treatment with NMDI14 results in no decrease in cell counts, demonstrating that the pharmacological inhibition of NMD can be achieved without subtle changes in proliferation. 941 genes are increased >1.5 fold with NMDI14. The treatment of N417 cells with NMDI14 for 6 hours leads to a steady state expression of p53 similar to that seen in U2OS cells. NMDI14 significantly increases the stability of PTC mutated p53 mRNA in N417 cells, without altering the stability of wild-type p53 in NMDI treated U2OS cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:NMD

Specifications

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

NMDI14 is a nonsense mediated RNA decay ( NMD ) inhibitor. NMDI14 disrupts the SMG7-UPF1 interactions and inhibits NMD .

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Isomeric SMILES: CCOC(=O)C1=C(SC(=C1C)C)NC(=O)CC2C(=O)NC3=C(N2)C=C(C(=C3)C)C
• PubChem CID: 2886617
• Molecular Weight: 415.51