PAK4 Mouse mAb - 1차 항체, specific to PAK4, Mouse IgG1

Cat. No.: Ab120121

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

K1E023_12G11

Conjugation

Unconjugated

개요

Mouse anti Human PAK4 Antibody, Monoclonal (K1E023_12G11), could be used for WB, IHC, IP and so on.

Specifications

Product Name

PAK4 Mouse mAb - 1차 항체, specific to PAK4, Mouse IgG1

동의어

EC 2.7.11.1 | p21-activated kinase 4 | PAK-4 | KIAA1142 antibody | p21 activated kinase 4 antibody | p21 Cdc42/Rac1-actiated kinase 4 antibody | P21 protein (Cdc42/Rac) activated kinase 4 antibody | p21(CDKN1A) activated kinase 4 antibody | p21-activated

사양 및 순도

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

생화학 및 생리적 메커니즘

Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/de

숙주 종

마우스

특이성

PAK4

면역원

Recombinant human PAK4 (AA 101-220).

포지티브 제어

WB: HEK293, Jurkat, HeLa, MCF7, LNCaP, NIH/3T3 and C6 cell lysates. IHC: Human prostate tissue. IP: HEK293 lysates.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -80°C,Avoid repeated freezing and thawing

배송

Dry ice packs + Cold packs

안정성 및 스토리지

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: PAK4 Mouse mAb (Ab120121)

PAK4 Mouse mAb (Ab120121) - Western Blot
All lanes: PAK4 Mouse mAb (Ab120121) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 74, 75 kDa
PAK4 can function as an antiapoptotic protein (PMID:14517283). And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
Exposure time: 2.9 s

IP: PAK4 Mouse mAb (Ab120121)

PAK4 Mouse mAb (Ab120121) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of PAK4 Mouse mAb (Ab120121) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PAK4 Mouse mAb (Ab120121) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: HEK293 lysate
Lane 2: PAK4 immunoprecipitated from HEK293 lysate by PAK4 Mouse mAb (Ab120121)
Lane 3: The same as Lane 2 but mouse IgG was used as IgG isotype control antibody
Result: PAK4 Mouse mAb (Ab120121) can immunoprecipitate PAK4.

IHC: PAK4 Mouse mAb (Ab120121)

PAK4 Mouse mAb (Ab120121) - IHC
IHC analysis of prostate tissue by PAK4 Mouse mAb (Ab120121).
IHC was performed using sections of the formalin-fixed paraffin-embedded prostate tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PAK4 Mouse mAb (Ab120121) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

적용

• 적용: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

• IHC:

  1/200 ;

관련 대상(인간)

PAK4 Tchem Serine/threonine-protein kinase PAK 4 (0 Activities)

Discover Target: PAK4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PAK4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.