Periodic Acid Solution (1%) - BioReagent, Biological Stain, for Microscopy, 1% (w/v) in water

Cat. No.: P774826

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 1% (w/v) in water

Synonyms

1% Periodic Acid Aqueous Solution | Periodic Acid Reagent (1%)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

개요

  Periodic acid (also known as periodic acid) is a strong oxidizing agent. It oxidizes the 1,2-glycol groups in carbohydrates and related substances to form dialdehydes. The aldehydes react with Schiff reagent to form a fuchsin compound, producing a purplish-red color. Therefore, periodic acid is often used in combination with Schiff reagent for glycogen staining. Since periodic acid can also oxidize other intracellular substances, care should be taken to select the appropriate concentration of periodic acid and oxidation time. The oxidation should be controlled to oxidize glycol groups to aldehyde groups without causing over-oxidation, which is a critical step. Periodic acid solution is suitable for thin tissue sections, especially for oxidizing cultured cells.

Protocol

1. Perform routine fixation, usually with 10% formalin, followed by routine dehydration and embedding.
2. Deparaffinize paraffin sections and transfer to distilled water; directly place frozen sections into distilled water.
3. Rinse twice with distilled water.
4. Incubate in periodic acid solution at room temperature for 5–8 min, generally not exceeding 10 min. Follow the specific requirements of the experiment for subsequent steps.

Precautions

1. Deparaffinization of sections should be thorough; otherwise, it will affect the staining result. Staining time for frozen sections should be as short as possible.
2. The oxidation time with periodic acid should not be too long; the optimal temperature for oxidation is 18–22°C.
3. Store sealed at 4°C, and avoid excessive exposure to sunlight and air during use. Before use, it is best to take it out 30 minutes in advance to equilibrate to room temperature, and use it in a dark place protected from light.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

동의어

1% Periodic Acid Aqueous Solution | Periodic Acid Reagent (1%)

사양 및 순도

BioReagent, Biological Stain, for Microscopy, 1% (w/v) in water

안정성 및 스토리지

Store at 2-8℃ long term (12 months). Store in the dark.

보관 조건

Store at 2-8°C,Protected from light

배송

Wet ice

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등급

Biological Stain, BioReagent, 현미경용

화학 및 물리적 특성

• 감도: Light-sensitive