PTEN Mouse mAb - 1차 항체, specific to PTEN, Mouse IgG1

Cat. No.: Ab123795

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IHC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

C3

Conjugation

Unconjugated

개요

Mouse anti Human PTEN Antibody, Monoclonal(C3), could be used for WB, IHC and so on .
Application:
WB: 0.01-3 µg/mL
IHC: 5-20 µg/mL
Protein Function
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.

Specifications

Product Name

PTEN Mouse mAb - 1차 항체, specific to PTEN, Mouse IgG1

동의어

PTEN | MMAC1 | TEP1 | Phosphatidylinositol 3 | 4 | 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN | Mutated in multiple advanced cancers 1 | Phosphatase and tensin homolog | 10q23del antibody | BZS antibody | DEC antibody | GL

사양 및 순도

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

숙주 종

마우스

특이성

PTEN

면역원

Recombinant Human PTEN expressed in E.coli (AA 2-403)

포지티브 제어

WB: HeLa, HUVEC, MCF7, C6, NIH/3T3 and Raw264.7 cell lysates. IHC: Human cerebellum tissue.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A + Protein G purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: PTEN Mouse mAb (Ab123795)

PTEN Mouse mAb (Ab123795) - Western Blot
All lanes: PTEN Mouse mAb (Ab123795) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040)at 1/20000 dilution

Predicted band size: 54 kDa
Observed band size: 53 kDa
Exposure time: 300 s

IHC: PTEN Mouse mAb (Ab123795)

PTEN Mouse mAb (Ab123795) - IHC
DAB staining on IHC
Sample: Human Cerebellum Tissue
Primary Ab: 20µg/mL PTEN Mouse mAb (Ab123795)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: PTEN Mouse mAb (Ab123795)

PTEN Mouse mAb (Ab123795) - IHC
DAB staining on IHC
Sample: Human Cerebrum Tissue
Primary Ab: 20µg/mL PTEN Mouse mAb (Ab123795)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

적용

• 적용: Dilution info
• WB:

  0.01-3 µg/mL;

• IHC:

  5-20 µg/mL;

관련 대상(인간)

PTEN Tchem Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (0 Activities)

Discover Target: PTEN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PTEN Tchem Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (4 Activities)

Discover Target: PTEN

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PTEN Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.