Rad9 Mouse mAb - 1차 항체, specific to Rad9, Mouse IgG2b

Cat. No.: Ab124408

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IP, WB

Host species

마우스

Reactive species

마우스, 원숭이, 인간, 조언

Isotype

Mouse IgG2b

Clone Number

3A3-A7-F8

Conjugation

Unconjugated

개요

Mouse anti Human Rad9 Antibody, Monoclonal (3A3-A7-F8), could be used for WB, IP and so on.
Application:
WB: 1/500-1/1000
IP: 1/20
Protein Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Specifications

Product Name

Rad9 Mouse mAb - 1차 항체, specific to Rad9, Mouse IgG2b

동의어

DNA repair exonuclease rad9 homolog A | EC 3.1.11.2 | hRAD9 | Cell cycle checkpoint control protein antibody | Cell cycle checkpoint control protein RAD9A antibody | DNA repair exonuclease rad9 homolog A antibody | hRAD 9 antibody | hRAD9 antibody | Rad 9

사양 및 순도

ExactAb™, Validated, 1.0 mg/mL

숙주 종

마우스

특이성

Rad9

면역원

A synthetic peptide derived from human RAD9A (AA 264-370)

포지티브 제어

WB: HeLa, MCF7, NIH/3T3 and C6 cell lysates. IP: HeLa and NIH/3T3 cell lysates.

변화

Unconjugated

등급

ExactAb™, Validated

아이소타입

Mouse IgG2b

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

1.0 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant RPL7A Antibody (Ab125864)

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 49 kDa
Exposure time: 16.2 s

Western Blot: Recombinant RPL7A Antibody (Ab125864)

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 20.0 s

IP: Rad9 Mouse mAb (Ab124408)

Rad9 Mouse mAb (Ab124408) - IP
Immunoprecipitation analysis of Rad9 in HeLa and NIH/3T3 lysates using Rad9 Mouse mAb (Ab124408).

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

• IP:

  1/20;

관련 대상(인간)

RAD9A Tbio Cell cycle checkpoint control protein RAD9A (0 Activities)

Discover Target: RAD9A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)