GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes.
Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens.
Activates signaling pathways that lead to
숙주 종
마우스
특이성
Human CD19
면역원
NALM1 + NALM16 cells
포지티브 제어
Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
변화
AF405
여기(nm)
Violet Laser(405nm)
방출(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
등급
ExactAb™, Recombinant, Validated
라이트 체인 유형
kappa
정화 방법
Protein A purified
집중력
5 μL/test
보관 및 배송
보관 조건
Store at 2-8°C,Protected from light,Do not freeze
배송
Wet ice,Do not freeze
안정성 및 스토리지
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
이미지
Recombinant CD19 Antibody (AF405) (Ab223516) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (AF405) (Ab223516) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).
Recombinant CD19 Antibody (AF405) (Ab223516) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (AF405) (Ab223516) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD19 Antibody (AF405) (Ab223516) - Flow Cytometry Flow cytometry analysis of CD19 expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (AF405) (Ab223516) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.
적용
적용
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD19 Antibody (AF405)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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