Recombinant eIF4E Antibody - 1차 항체, specific to EIF4E, Rabbit IgG

Cat. No.: Ab101380

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human eIF4E Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Specifications

Product Name

Recombinant eIF4E Antibody - 1차 항체, specific to EIF4E, Rabbit IgG

동의어

CBP | eIF4E | eIF-4E | EIF4E1 | EIF4EL1 | EIF4EL1MGC111573 | eIF-4F 25 kDa subunit | EIF4F | eukaryotic translation initiation factor 4E | eukaryotic translation initiation factor 4E-like 1 | mRNA cap-binding protein | AUTS19 antibody | CBP antibody | eIF

사양 및 순도

ExactAb™, Validated, Recombinant, 0.5 mg/mL

숙주 종

토끼

특이성

EIF4E

면역원

A synthetic peptide derived from human eIF4E (AA 176-194)

포지티브 제어

WB: HeLa, K562, A431, HEK293, C6 and NIH/3T3 lysates. IHC: Mouse colon tissue. IF/ICC: HeLa cells.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Immunogen affinity purified

집중력

0.5 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - Western Blot
All lanes: Recombinant eIF4E Antibody (Ab101380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21.2 s

IHC: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant eIF4E Antibody (Ab101380). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - IF/ICC

IF analysis of Recombinant eIF4E Antibody (Ab101380) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF4E Antibody (Ab101380) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

관련 대상(인간)

EIF4E Tchem Eukaryotic translation initiation factor 4E (0 Activities)

Discover Target: EIF4E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

EIF4E Tchem Eukaryotic translation initation factor (600 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EIF4E Tchem Eukaryotic translation initiation factor 4E/Eukaryotic translation initiation factor 4E-binding protein 1 (15 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EIF4E Tchem EIF4E/EIF4G1 (7 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF4E Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.