GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.6 mg/mL
ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Rabbit anti Human LIN28B Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Function Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Specifications
Product Name
Recombinant LIN28B Antibody - 1차 항체, specific to LIN28B, Rabbit IgG
동의어
CSDD 2 antibody | CSDD2antibody | FLJ16517 antibody | Lin 28 homolog B (C. elegans) antibody | Lin 28.2 antibody | Lin-28B antibody | LIN28B antibody | LN28B_HUMAN antibody | Protein lin-28 homolog B antibody | CSDD2 | CSDD2protein lin-28 homolog B | FLJ1
사양 및 순도
ExactAb™, Validated, Recombinant, 0.6 mg/mL
숙주 종
토끼
특이성
LIN28B
면역원
A synthetic peptide derived from human Lin28B (AA 1-250)
포지티브 제어
WB: HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: MCF7 cells.
변화
Unconjugated
등급
ExactAb™, Recombinant, Validated
아이소타입
Rabbit IgG
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A purified
집중력
0.6 mg/mL
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
Recombinant LIN28B Antibody (Ab113176) - Western Blot All lanes: Recombinant LIN28B Antibody (Ab113176) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 33 kDa Exposure time: 23.1 s
Recombinant LIN28B Antibody (Ab113176) - IF/ICC Immunofluorescence analysis of LIN28B in MCF7 using Recombinant LIN28B Antibody (Ab113176).
적용
적용
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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