Recombinant SNAP25 Antibody - 1차 항체, specific to SNAP25, Rabbit IgG

Cat. No.: Ab128454

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.4 mg/mL

Application

IF/ICC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human SNAP25 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

Specifications

Product Name

Recombinant SNAP25 Antibody - 1차 항체, specific to SNAP25, Rabbit IgG

동의어

bA416N4.2 antibody | Bdr antibody | CMS18 antibody | dJ1068F16.2 antibody | FLJ23079 antibody | HGNC:11132 antibody | MGC105414 antibody | MGC139754 antibody | Resistance to inhibitors of cholinesterase 4 homolog antibody | RIC 4 antibody | RIC-4 antibody

사양 및 순도

ExactAb™, Validated, Recombinant, 0.4 mg/mL

숙주 종

토끼

특이성

SNAP25

면역원

A synthetic peptide derived from human SNAP25 (AA 193-206)

포지티브 제어

WB: Mouse brain lysates. ICC/IF: HeLa cells.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified

집중력

0.4 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant SNAP25 Antibody (Ab128454)

Recombinant SNAP25 Antibody (Ab128454) - Western Blot
All lanes: Recombinant SNAP25 Antibody (Ab128454) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 2.7 s

IF: Recombinant SNAP25 Antibody (Ab128454)

Recombinant SNAP25 Antibody (Ab128454) - IF
Immunocytochemistry analysis of SNAP25 (green) in Hela using Recombinant SNAP25 Antibody (Ab128454), and DAPI (blue).

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

관련 대상(인간)

SNAP25 Tclin Synaptosomal-associated protein 25 (0 Activities)

Discover Target: SNAP25

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant SNAP25 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.