Recombinant STAT3 Antibody - 1차 항체, specific to STAT3, Rabbit IgG

Cat. No.: Ab129421

GRADE & PURITY Recombinant, Validated, ExactAb™, KD Validation, See COA

Application

IF/ICC, IHC, WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human STAT3 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. 

Specifications

Product Name

Recombinant STAT3 Antibody - 1차 항체, specific to STAT3, Rabbit IgG

동의어

APRFMGC16063 | DNA-binding protein APRF | FLJ20882 | HIES | signal transducer and activator of transcription 3 (acute-phase responsefactor) | signal transducer and activator of transcription 3 | STAT3 | 1110034C02Rik antibody | Acute-phase response factor

사양 및 순도

Recombinant, ExactAb™, KD Validation, Validated, See COA

생화학 및 생리적 메커니즘

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:173442

숙주 종

토끼

특이성

STAT3

면역원

A synthetic peptide derived from human STAT3 (AA 670-700)

포지티브 제어

WB: Mouse heart, HT-1080, HeLa, HEK293, A375, A431, NIH/3T3 and C6 lysates. IHC: Rat brain, Human astrocytoma, Human Hodgkin's lymphoma tissues. IF/ICC: HeLa cells.

변화

Unconjugated

등급

ExactAb™, KD Validation, Recombinant, Validated

아이소타입

Rabbit IgG

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - Western Blot
All lanes: Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 91 kDa
Exposure time: 5.2 s

Western Blot: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - Western Blot
All lanes use Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution for 1 hour at room temperature.

IF/ICC: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - IF/ICC
Immunofluorescent analysis of Hela cells treated with IFN alpha, using Recombinant STAT3 Antibody (Ab129421).

IHC: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - IHC
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution. 

IHC: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - IHC
Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution.

IHC: Recombinant STAT3 Antibody (Ab129421)

Recombinant STAT3 Antibody (Ab129421) - IHC
Immunohistochemical analysis of paraffin-embedded rat brain, using Recombinant STAT3 Antibody (Ab129421).

적용

• 적용: Dilution info
• IHC:

  1/100 - 1/200;

• IF/ICC:

  1/50 - 1/200;

• WB:

  1/1000-1/2000 ;

관련 대상(인간)

STAT3 Tchem Signal transducer and activator of transcription 3 (0 Activities)

Discover Target: STAT3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

STAT3 Tchem Signal transducer and activator of transcription 3 (3313 Activities)

Discover Target: STAT3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

STAT3 Tchem PTPN9/STAT3 (12 Activities)

Discover Target: STAT3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant STAT3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.