Recombinant TRIM25 Antibody - 1차 항체, specific to TRIM25, Rabbit IgG

Cat. No.: Ab132484

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

토끼

Reactive species

인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human TRIM25 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Specifications

Product Name

Recombinant TRIM25 Antibody - 1차 항체, specific to TRIM25, Rabbit IgG

동의어

2.3.2.27 | EC 6.3.2.n3 | Estrogen-responsive finger protein | RING finger protein 147 | RING-type E3 ubiquitin transferase | RING-type E3 ubiquitin transferase TRIM25 | Tripartite motif-containing protein 25 | Ubiquitin/ISG15-conjugating enzyme TRIM25 | Z

사양 및 순도

ExactAb™, Validated, Recombinant, 0.5 mg/mL

숙주 종

토끼

특이성

TRIM25

면역원

A synthetic peptide derived from human TRIM25 (AA 142-157)

포지티브 제어

WB: K562, HeLa and C6 cell lysates. ICC/IF: MCF7 cells.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified

집중력

0.5 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant TRIM25 Antibody (Ab132484)

Recombinant TRIM25 Antibody (Ab132484) - Western Blot
All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 71 kDa
Observed band size: 69 kDa
Exposure time: 17.0 s

Western Blot: Recombinant TRIM25 Antibody (Ab132484)

Recombinant TRIM25 Antibody (Ab132484) - Western Blot
All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 71 kDa
Observed band size: 69 kDa
Exposure time: 1.0 s

IF/ICC: Recombinant TRIM25 Antibody (Ab132484)

Recombinant TRIM25 Antibody (Ab132484) - IF/ICC
Immunocytochemistry analysis of TRIM25/EFP (green) in MCF7 using Recombinant TRIM25 Antibody (Ab132484), and cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

관련 대상(인간)

TRIM25 Tbio E3 ubiquitin/ISG15 ligase TRIM25 (0 Activities)

Discover Target: TRIM25

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant TRIM25 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.