RSL3 - 10mM in DMSO , CAS No.1219810-16-8

CAS: 1219810-16-8 Cat. No.: R407911 분자식: C₂₃H₂₁ClN₂O₅ 분자량: 440.88 PubChem CID: 1750826

GRADE & PURITY 10mM in DMSO

Synonyms

(1S,3R)-RSL3 | 1H-​Pyrido[3,​4-​b]​indole-​3-​carboxylic acid, 2-​(2-​chloroacetyl)​-​2,​3,​4,​9-​tetrahydro-​1-​[4-​(methoxycarbonyl)​phenyl]​-​, methyl ester, (1S,​3R)​-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

개요

Information

RSL3 RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
In vitro

Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3\'s ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment.

In vivo

Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice.
Cell Data

cell lines：MOLT-4 cell lines and Reh (pre-B cells)

Concentrations：1 µg/ml

Incubation Time：16hrs

Powder Purity:≥99%

Specifications

동의어

(1S,3R)-RSL3 | 1H-​Pyrido[3,​4-​b]​indole-​3-​carboxylic acid, 2-​(2-​chloroacetyl)​-​2,​3,​4,​9-​tetrahydro-​1-​[4-​(methoxycarbonyl)​phenyl]​-​, methyl ester, (1S,​3R)​-

사양 및 순도

10mM in DMSO

생화학 및 생리적 메커니즘

RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.

보관 조건

Store at -80°C

배송

Dry ice packs + Cold packs

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작업 유형

ACTIVATOR

이름과 식별자

• 이성체 SMILES: COC(=O)[C@H]1CC2=C([C@@H](N1C(=O)CCl)C3=CC=C(C=C3)C(=O)OC)NC4=CC=CC=C24
• PubChem CID: 1750826
• 분자량: 440.88

관련 대상(인간)

GPX4 Tchem Phospholipid hydroperoxide glutathione peroxidase (1 Activities)

Discover Target: GPX4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

참고 문헌

1. Xiaosi Lin, Le Dong, Qing Miao, Zhaobin Huang, Fang Wang.  (2024)  Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines.  EUROPEAN JOURNAL OF PHARMACOLOGY,      [PMID:38663542] [10.1016/j.ejphar.2024.176608]

2. Haoze Song, Jing Liang, Yuanyuan Guo, Yang Liu, Kuiru Sa, Guohong Yan, Wen Xu, Wei Xu, Lixia Chen, Hua Li.  (2023)  A potent GPX4 degrader to induce ferroptosis in HT1080 cells.  EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:38194774] [10.1016/j.ejmech.2023.116110]

3. Zhao Hongting, Zhang Meng, Zhang Jinghua, Sun Zichen, Zhang Wenxin, Dong Weichen, Cheng Chen, Yao Yongzhong, Li Kuanyu.  (2023)  Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth.  Cell and Bioscience,  13  (1): (1-15).  [PMID:37179385] [10.1186/s13578-023-01044-0]

4. Yu-Chen Chen, Qiong Zheng, Hui Lu, Yi-Xin Zhuang, Ting-Ting Gong, Lie-Feng Ma, Zha-Jun Zhan.  (2023)  Neuroprotective flavonoids from Epimedium brevicornu by inhibition of ferroptosis.  Phytochemistry Letters,      [PMID:] [10.1016/j.phytol.2023.04.003]

5. Fan Zhang, Hualong Zhen, Hengshun Cheng, Fengying Hu, Yunfei Jia, Binbin Huang, Minmin Jiang.  (2022)  Di-(2-ethylhexyl) phthalate exposure induces liver injury by promoting ferroptosis via downregulation of GPX4 in pregnant mice.  Frontiers in Cell and Developmental Biology,      [PMID:36438553] [10.3389/fcell.2022.1014243]

6. Chao Zhao, Xiaoyan Huang, Zhuoyang Wu, Wanlu Sun, Yi Pan, Yi Liu.  (2025)  pH-Responsive Self-Assembled Upconversion Photosensitizer and RSL3 Synergistically Induce Tumor Ferroptosis.  JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:40665790] [10.1021/acs.jmedchem.5c01339]

7. Cong Zhang, Yangkun Xiong, Mingyang Gong, Haixia Zhao, Shan Li, Xin Chen, Xiaochuan Ye, Zhenpeng Qiu.  (2025)  Pachymic Acid Ameliorates Fructose-Driven Hyperuricemic Nephropathy in Mice by Suppressing Ferroptosis via Activating Nrf2/GPX-4 Pathway.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:40864644] [10.1021/acs.jafc.5c02398]

8. Xue Shi, Yun Zhao, Hong-Yu Gao, Wei Yang, Jun Liao, Hui-Han Wang, Xiao-Tian Wang, Wei Yan.  (2025)  NFS1, together with FXN, protects cells from ferroptosis and DNA damage in diffuse large B-cell lymphoma.  Redox Biology,      [PMID:41005206] [10.1016/j.redox.2025.103878]

9. Wuyang Shi, Shibo Sun, Haowen Liu, Yao Meng, Kangshuai Ren, Guoying Wang, Minghui Liu, Jiaqi Wu, Yue Zhang, Huang Huang, Meiyun Shi, Weiping Xu, Qiang Ma, Bingbing Sun, Jianqiang Xu.  (2024)  Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif.  Redox Biology,      [PMID:38277963] [10.1016/j.redox.2024.103050]

10. Weili Wu, Yingying Zhao, Baifu Qin, Xin Jiang, Chuyue Wang, Rong Hu, Rui Ma, Mong-Hong Lee, Huanliang Liu, Kai Li, Ping Yuan.  (2023)  Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer.  EBioMedicine,      [PMID:37343364] [10.1016/j.ebiom.2023.104650]

11. Yi-Xuan Zeng, Ni-Ren Li, Bing-Ying Deng, Yu-Feng Gu, Si-Fan Lu, Yi Liu.  (2025)  Coix Seed Oil Alleviates DSS-Induced Ulcerative Colitis via Intestinal Barrier Repair and Ferroptosis Regulation.  Journal of Inflammation Research,      [PMID:39995826] [10.2147/JIR.S501745]

12. Zi-Han Xu, Ming-Min Xie, Chun-Lan Xie, Xian-Wen Yang, Jun-Song Wang.  (2025)  Deep-Sea-Derived Isobisvertinol Targets TLR4 to Exhibit Neuroprotective Activity via Anti-Inflammatory and Ferroptosis-Inhibitory Effects.  Marine Drugs,  23  (1): (49).  [PMID:39852551] [10.3390/md23010049]

13. Deshan Yao, Liuxiang Bao, Sichuan Wang, Meng Tan, Yuanyuan Xu, Tianxu Wu, Zhengang Zhang, Kaizheng Gong.  (2024)  Isoliquiritigenin alleviates myocardial ischemia-reperfusion injury by regulating the Nrf2/HO-1/SLC7A11/GPX4 axis in mice.  FREE RADICAL BIOLOGY AND MEDICINE,      [PMID:38734270] [10.1016/j.freeradbiomed.2024.05.012]

14. Huiqin Zhou, Yunjia Peng, Xinhua Huo, Bingqing Li, Huasheng Liu, Jian Wang, Gaihua Zhang.  (2025)  Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer’s Disease.  Journal of Inflammation Research,      [PMID:39959647] [10.2147/JIR.S497418]

15. Weng Xiuhong, Wang Simin, Wang Qing, Wei Mingbo, Cheng Bo.  (2025)  Melatonin inhibits salivary gland epithelial cell ferroptosis via the NRF2/HO-1/GPX4 signaling pathway in primary Sjögren’s syndrome.  INFLAMMATION RESEARCH,  74  (1): (1-19).  [PMID:40413364] [10.1007/s00011-025-02047-y]

16. Jiajian Wang, Lanqing Zhang, Runfang Li, Rui Yang, Fan Yang, Fei Yu, Jihong Zhang.  (2025)  Gambogic acid restores wild-type-like p53 functions to induce ferroptosis in mutant p53 cancer cells.  BIOCHEMICAL PHARMACOLOGY,      [PMID:41213406] [10.1016/j.bcp.2025.117533]

17. Weichen Dong, Wenxin Zhang, Li Huang, Lulu Xiao, Yuanfei Luo, Linying Yuan, Yike Jiang, Anyu Liao, Hongting Zhao, Zhihui Liu, Jia Wang, Ying Zhao, Yulong Cai, Mengna Peng, Dong Yang, Yi Xie, Kuanyu Li, Wusheng Zhu.  (2026)  Restoring cardiolipin homeostasis mitigates cerebral ischemia–reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis.  Journal of Advanced Research,      [PMID:] [10.1016/j.jare.2026.03.014]

18. Ke-xin Cao, Zhi-yuan Xia, Yuan-zhi Cheng, Yan-ping Feng, Meng Liu, Jia-cheng Yang, Yu-xuan Huang, Zhang-chao Deng, Gang Zuo, Xin Gen Lei, Lv-hui Sun.  (2026)  Nano-Selenium Alleviates Heat Stress-Induced Testicular Injury in Roosters via Inhibiting Ferroptosis.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:42381562] [10.1021/acs.jafc.6c06484]