등급 & 순도ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.5 mg/mL
ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Mouse anti Human S100A10 Antibody, Monoclonal (6F4E6D5C10), could be used for WB, IF/ICC and so on.
사양
Product Name
S100A10 Mouse mAb - 1차 항체, specific to S100A10, Rabbit IgG
동의어
Calpactin I light chain | Calpactin-1 light chain | Cellular ligand of annexin II | p10 protein | p11 | S100 calcium-binding protein A10 | 42C antibody | AA409961 antibody | AL024248 antibody | Annexin II ligand antibody | Annexin II ligand, calpactin I,
사양 및 순도
ExactAb™, Validated, 0.5 mg/mL
생화학 및 생리적 메커니즘
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
숙주 종
마우스
특이성
S100A10
면역원
Recombinant protein of mouse S100A10.
포지티브 제어
WB: HeLa, A431, TH-29, A549, HUVEC and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
변화
Unconjugated
등급
ExactAb™, Validated
아이소타입
Rabbit IgG
SDS-PAGE
150 kDa
정화 방법
Immunogen affinity purified
집중력
0.5 mg/mL
Product Line
생물 제품
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
S100A10 Mouse mAb (Ab126219) - Western Blot All lanes: S100A10 Mouse mAb (Ab126219) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 13 kDa Exposure time: 58.4 s
S100A10 Mouse mAb (Ab126219) - IF/ICC Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100A10 Mouse mAb (Ab126219) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.