Smad3 Mouse mAb - 1차 항체, specific to Smad3, Mouse IgG2b

Cat. No.: Ab188096
주문 가능
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
마우스
Reactive species
마우스, 인간, 조언
Isotype
Mouse IgG2b
Clone Number
FG01/2C1
Conjugation
Unconjugated
★
Size
USA
독일 (EU)*
Price
Qty
10μl
Ab188096-10μl
재고 있음 ≥10
—
US$79.90
50μl
Ab188096-50μl
7 재고 있음
—
US$259.90
100μl
Ab188096-100μl
2 재고 있음
—
US$419.90
1ml
Ab188096-1ml
US 주문제작 · 2–4주 ·
—
US$2,799.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

WB: 1/1000 – 1/100,000

Flow: 1/200 - 1/2,000

ICC/IF: 1/200

Specifications

Product Name
Smad3 Mouse mAb - 1차 항체, specific to Smad3, Mouse IgG2b
동의어
DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
사양 및 순도
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
숙주 종
마우스
특이성
Smad3
면역원
Recombinant full length protein corresponding to Human Smad3
포지티브 제어
WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells ICC/IF: U-2 OS cells
변화
Unconjugated
등급
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
아이소타입
Mouse IgG2b
라이트 체인 유형
kappa
SDS-PAGE
150.0 KDa
정화 방법
Protein A purified
집중력
1.0 mg/mL
보관 및 배송
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
이미지

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 49 kDa
Exposure time: 0.9 s 

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 29.6 s 

Samd3 Mouse mAb (Ab188096) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Samd3 Mouse mAb (Ab188096) - ICC/IF
IF analysis of Samd3 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Samd3 Mouse mAb (Ab188096) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

적용
적용Dilution info
Flow Cytometry

1/200-1/2000

IF/ICC

1/200

WB

1/1000 – 1/100,000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
작용 메커니즘
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate Type날짜항목
ZJ24F0607491Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607492Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607493Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24R0600677Certificate of AnalysisJun 14, 2024 Ab188096
자주 묻는 질문과 기사
솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

관련 상품

Goat Anti-Mouse IgG H&L Antibody - 2차 항체, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - 아이소타입 대조 항체, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - 2차 항체, IgG
BALB/c Mouse IgG - 아이소타입 대조 항체, IgG
Rabbit Anti-Mouse IgG antibody - 2차 항체
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체
Recombinant SMAD3 Antibody - 1차 항체, specific to SMAD3, Rabbit IgG
Goat Anti-Mouse IgG H&L Antibody - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체
Rabbit Anti-Mouse IgG (HRP) - 2차 항체
Rabbit Anti-Mouse IgG (Biotin) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 2차 항체, Goat IgG
Mouse IgG (Biotin) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (PE) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (APC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (FITC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG2a-Fc - 아이소타입 대조 항체, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 2차 항체, Goat IgG
Smad3 Mouse mAb - 1차 항체, specific to Smad3, Mouse IgG1
Smad3 Mouse mAb - 1차 항체, specific to Smad3, Mouse IgG2b
Mouse IgG2b-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - 아이소타입 대조 항체, IgG
Mouse IgG (PerCP) - 아이소타입 대조 항체, IgG
Mouse IgG (AF405) - 아이소타입 대조 항체, IgG
Mouse IgG (AF647) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF555) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF700) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - 아이소타입 대조 항체
Mouse IgG (PE-Cy7) - 아이소타입 대조 항체
마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L
마우스 IgG2a-카파, 항-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - 아이소타입 대조 항체
Mouse IgG2a-Fc (FITC) - 아이소타입 대조 항체
Mouse IgG (AF555) - 아이소타입 대조 항체
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - 2차 항체, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 1차 항체, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - 2차 항체
Goat Anti-Mouse IgG H&L (PE-Cy7) - 2차 항체
Mouse IgG (PE-Cy5.5) - 아이소타입 대조 항체
Goat Anti-Mouse IgG H&L (AF750) - 2차 항체
Mouse IgG2a-kappa (AF555), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - 아이소타입 대조 항체, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - 2차 항체
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 2차 항체
Goat Anti-Mouse IgG H&L (Cy3) - 2차 항체
Mouse IgG2a-kappa (APC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG (PE-AF647) - 아이소타입 대조 항체

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