SQSTM1 Mouse mAb - 1차 항체, specific to SQSTM1, Mouse IgG1

Cat. No.: Ab129090

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

마우스

Reactive species

마우스, 인간

Isotype

Mouse IgG1

Clone Number

C1

Conjugation

Unconjugated

개요

Mouse anti Human SQSTM1 Antibody, Monoclonal (C1), could be used for WB, IF/ICC, IHC, Flow Cytometry and so on.

Specifications

Product Name

SQSTM1 Mouse mAb - 1차 항체, specific to SQSTM1, Mouse IgG1

동의어

A170 | Autophagy Receptor P62 | DMRV | EBI3-associated protein of 60 kDa | EBI3-associated protein p60 | EBIAP | FTDALS3 | NADGP | ORCA | OSIL | oxidative stress induced like | p60PDB3 | p62 | p62B | Paget disease of bone 3 | PDB3 | phosphotyrosine indepe

사양 및 순도

Carrier Free, ExactAb™, Validated, High Performance, See COA

생화학 및 생리적 메커니즘

Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitina

숙주 종

마우스

특이성

SQSTM1

면역원

Recombinant SQSTM1 expressed in E.coli (AA 136-400).

포지티브 제어

WB: HeLa and A549 cell lysates. IF/ICC: HeLa cells. IHC: Human breast cancer tissue. Flow Cytometry: A549 cells.

변화

Unconjugated

등급

Carrier Free, ExactAb™, High Performance, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A + Protein G purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - Western Blot
All lanes: SQSTM1 Mouse mAb (Ab129090) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 54 kDa
Exposure time: 1.5 s

IF: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - IF
Immunocytochemistry analysis of SQSTM1/p62 (green) in U87-MG cells using SQSTM1 Mouse mAb (Ab129090), and cells were counterstained with DAPI (blue).

IHC: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SQSTM1 Mouse mAb (Ab129090). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Flow Cytometry: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - Flow Cytometry
Human A549 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-TritonX-100, then stained with 10 µg/mL SQSTM1 Mouse mAb (Ab129090, red histogram) or Isotype control antibody (green histogram), followed by 1µg/mL FITC conjugated Anti-mouse IgG Secondary Antibody.

적용

적용

Dilution info

WB

0.01-2 µg/mL

IHC

2-20 µg/mL

Flow Cytometry

10µg/mL

관련 대상(인간)

SQSTM1 Tbio Sequestosome-1 (0 Activities)

Discover Target: SQSTM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SQSTM1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.