BioReagent, HEPES‑containing BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Balanced Salt Solution (BSS) matches the pH, osmotic pressure and other environmental conditions under cell‑growth status. It can maintain osmotic pressure, regulate acid‑base balance, and supply energy and inorganic salts essential for cell survival and metabolism, satisfying the basic requirements for cell survival and certain metabolic activities in in‑vitro experiments.
Modified Tyrode's solution powder is one type of balanced salt solution. It is mainly composed of sodium chloride, phosphate, calcium chloride, glucose, etc., and contains HEPES. Its buffering capacity is stronger than that of conventional Tyrode's solution powder (Aladdin Cat. No.: T1529491). In this product, calcium chloride is stored separately from other components. Dissolve them respectively and mix prior to use to avoid insoluble precipitates caused by simultaneous dissolution. It is frequently used for mammalian organ perfusion, tissue rinsing, and maintaining the normal physiological functions of isolated intestinal muscle.
This reagent is for research use only and shall not be used for clinical diagnosis or other purposes.
Contents & Storage
| Component No. | Component Name | Appearance | 1L | Storage |
| M1529492A | Calcium‑free modified Tyrode's solution powder | Powder | 1EA | 2-8℃. |
| M1529492B | Calcium salt solution | Liquid | 1EA | 2-8℃. |
Operating Procedures (For reference only):
1. Fully dissolve 1‑L‑packaged calcium‑free modified Tyrode's solution powder in 800 mL deionized water in a 1‑L beaker. Add calcium salt solution, mix thoroughly and make up the volume to 1000 mL for preservation or rinsing of isolated tissues.
2. pH adjustment is generally unnecessary; adjust pH manually if needed. Valid for 6 months when stored at 4 °C.
Precautions:
1. Precipitates are liable to form due to calcium ions in modified Tyrode’s solution. Minor precipitates can be dissolved by heating or removed by filtration before use. Discard the solution in case of massive precipitates.
2. This reagent contains glucose and HEPES. Bacterial growth may occur if the prepared solution is improperly stored. Check the condition of the solution before use.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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