Ubiquitin E1 Enzyme (UBE1), human recombinant, CAS No.U388907

CAS: U388907 Cat. No.: U388907 EC 번호: 230-907-9

GRADE & PURITY EnzymoPure™, 0.5 μg/μl in PBS containing 0.01% sodium azide

개요

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.

Specifications

Product Name

Ubiquitin E1 Enzyme (UBE1), human recombinant, CAS No.U388907

등급

EnzymoPure™

사양 및 순도

EnzymoPure™, 0.5 μg/μl in PBS containing 0.01% sodium azide

CAS

U388907

분자 유형

Enzyme

보관 및 배송

집중력

0.5 μg/μl in PBS containing 0.01% sodium azide

보관 조건

Store at -80°C

배송

Dry ice packs + Cold packs