Alpha Galactosidase

Cat. No.: A489917

GRADE & PURITY Specific Activity ＞30 U/mg;Activity 80 U/ml

Bioactivity

400 U/ml

Overview

Product Description

Alpha Galactosidase from E. coli cleaves α(1-3)- and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. There is no activity on α(1-4) linked galactose. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with living cells.

Molecular Weight

~80,000 daltons

Contents

Alpha galactosidase in 50 mM sodium phosphate, pH 7.5

included with 20 µL and 60 µl pack sizes:

Reaction buffer - 250mM Sodium phosphate, pH 6.5

Specificity

Non-reducing terminal alpha-(1-3)- and alpha-(1-6)- galactose. There is no activity on alpha-(1-4)-galactose.

Stability

Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.

Specific Activity

One unit of alpha-(1-3,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 25°C pH 6.5 from p-nitrophenyl-alpha-D-galactopyranoside.

Purity

α(1-3,6) galactosidase is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37°C with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.

The production host strain has been extensively tested and does not produce any detectable glycosidases.of the BSA band after SDS-PAGE should show no evidence of degradation.

Directions for use

1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.

2. Add water to 13 µl and 4 µl 5X Reaction Buffer.

3. Add 2 µl alpha-(1-3,6)-Galactosidase.

4. Incubate at 37°C for 1 hour. Longer incubations are necessary if fucose is present on the penultimate sugar.

Applications

Structural analysis of oligosaccharides

Xenograft transplantation studies

Removing heterogeneity from glycoproteins

Specifications

Product Name

Alpha Galactosidase

Synonyms

α-D-galactoside galactohydrolase, melibiase

Specifications & Purity

Specific Activity ＞30 U/mg;Activity 80 U/ml

Bioactivity

400 U/ml

Enzyme Commission Number

3.2.1.22

Molecule Type

Enzyme

Storage and Shipping

Concentration

Specific Activity ＞30 U/mg;Activity 80 U/ml

Storage

Store at 2-8°C

Shipped In

Wet ice