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Biotinylation reagents

Explore biotinylation reagents for labeling, pull-down workflows, target capture, and assay development. Compare reactive biotin tools suited to protein, peptide, and other biomolecule modification.

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  1. N-Boc-2-Biotinamidoethylamine
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    N-Boc-2-Biotinamidoethylamine
    CAS: 225797-46-6 Formula: C17H30N4O4S Molecular Weight: 386.51
    for TLC ? TLC grade — purity suited to thin-layer chromatography development. Use for TLC mobile phases and spotting where clean separation matters.
    Out of Stock Item #: N293556
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    Technical Identifiers
    IUPAC Name
    tert-butyl N-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyl]carbamate
    SMILES
    CC(C)(C)OC(=O)NCCNC(=O)CCCCC1C2C(CS1)NC(=O)N2
    InChIKey
    DEUNEHFEIXQVDG-OBJOEFQTSA-N
    InChI
    1S/C17H30N4O4S/c1-17(2,3)25-16(24)19-9-8-18-13(22)7-5-4-6-12-14-11(10-26-12)20-15(23)21-14/h11-12,14H,4-10H2,1-3H3,(H,18,22)(H,19,24)(H2,20,21,23)/t11show more
    Synonyms
    N-Boc-Biotinylethylenediamine|225797-46-6|tert-Butyl (2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl...
  2. D-Biotin - Moligand™, ≥98%, CAS No. 58-85-5 by
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    D-Biotin
    CAS: 58-85-5 EC Number: 200-399-3 Formula: C10H16N2O3S Molecular Weight: 244.31
    Solid Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools. ≥98%
    In Stock Item #: B105433
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid
    SMILES
    [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
    InChIKey
    YBJHBAHKTGYVGT-ZKWXMUAHSA-N
    InChI
    1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
    Synonyms
    Bios II | 4bj8 | Acidum capobenicum [INN-Latin] | CHEBI:15956 | Hexahydro-2-oxo-1H-thieno(3,4-d)imidazole-4-pentanoic...
  3. 9-BiotinlaMino-4,7-dioxanonanoic acid N-hydroxysucciniMidyl ester - 97%, used for , CAS No. 596820-83-6, by Aladdin Scientific
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    9-BiotinlaMino-4,7-dioxanonanoic acid N-hydroxysucciniMidyl ester
    CAS: 596820-83-6 PubChem CID: 91757819 Formula: C21H32N4SO8 Molecular Weight: 500.56
    ≥97%
    In Stock Item #: B122232
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    Technical Identifiers
    IUPAC Name
    (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]propanoate
    SMILES
    C1CC(=O)N(C1=O)OC(=O)CCOCCOCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
    InChIKey
    UCJSJBIJPAZUGU-AVYPCKFXSA-N
    InChI
    1S/C21H32N4O8S/c26-16(4-2-1-3-15-20-14(13-34-15)23-21(30)24-20)22-8-10-32-12-11-31-9-7-19(29)33-25-17(27)5-6-18(25)28/h14-15,20H,1-13H2,(H,22,26)(H2,2show more
    Synonyms
    (+)-Biotin-PEG2-NHS Ester | N-Succinimidyl 9-(Biotinamido)-4,7-dioxanonanoate | Biotin-PEG2-NHS | Biotin-PEG2-SPA
  4. Biotin-PEG2-Amine - ≥97% CAS No(138529-46-1) by Aladdin Scientific
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    Biotin-PEG2-Amine
    CAS: 138529-46-1 Formula: C16H30N4SO4 Molecular Weight: 374.49
    Solid ≥97%
    In Stock Item #: B122226
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-(2-aminoethoxy)ethoxy]ethyl]pentanamide
    SMILES
    C1C2C(C(S1)CCCCC(=O)NCCOCCOCCN)NC(=O)N2
    InChIKey
    LWISPDYGRSGXME-YDHLFZDLSA-N
    InChI
    1S/C16H30N4O4S/c17-5-7-23-9-10-24-8-6-18-14(21)4-2-1-3-13-15-12(11-25-13)19-16(22)20-15/h12-13,15H,1-11,17H2,(H,18,21)(H2,19,20,22)/t12-,13-,15-/m0/s1
    Synonyms
    DTXSID301030316 | (+)-Biotin-PEG2-CH2CH2NH2 | AKOS027326131 | n-(8-amino-3,6-dioxaoctanyl) biotinamide | (+)-Biotin-(...
  5. Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester - 97%, used for , CAS No. 89889-52-1, by Aladdin Scientific
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    Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester(Biotin-XX NHS)
    CAS: 89889-52-1 Formula: C26H41N5O7S Molecular Weight: 567.70
    Solid ≥97%
    In Stock Item #: B122220
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    Technical Identifiers
    IUPAC Name
    (2,5-dioxopyrrolidin-1-yl) 6-[6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoylamino]hexanoate
    SMILES
    C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
    InChIKey
    ATYCFNRXENKXSE-MHPIHPPYSA-N
    InChI
    1S/C26H41N5O7S/c32-20(27-16-8-2-4-12-24(36)38-31-22(34)13-14-23(31)35)10-3-1-7-15-28-21(33)11-6-5-9-19-25-18(17-39-19)29-26(37)30-25/h18-19,25H,1-17H2show more
    Synonyms
    F16499 | MFCD00467154 | AC-23716 | A860997 | Biotin-XX-NHS | (2,5-dioxopyrrolidin-1-yl) 6-[6-[5-[(3aS,4S,6aR)-2-oxo-1...
  6. N-Biotinyl-N'-(3-maleimidopropionyl)-3,6-dioxaoctane-1,8-diamine
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    N-Biotinyl-N'-(3-maleimidopropionyl)-3,6-dioxaoctane-1,8-diamine
    CAS: 305372-39-8 EC Number: 678-247-4 Formula: C23H35N5O7S Molecular Weight: 525.62
    ≥95%
    In Stock Item #: B122231
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[3-(2,5-dioxopyrrol-1-yl)propanoylamino]ethoxy]ethoxy]ethyl]pentanshow more
    SMILES
    C1C2C(C(S1)CCCCC(=O)NCCOCCOCCNC(=O)CCN3C(=O)C=CC3=O)NC(=O)N2
    InChIKey
    UDPCXVIKHFVPSZ-HOIFWPIMSA-N
    InChI
    1S/C23H35N5O7S/c29-18(4-2-1-3-17-22-16(15-36-17)26-23(33)27-22)24-8-11-34-13-14-35-12-9-25-19(30)7-10-28-20(31)5-6-21(28)32/h5-6,16-17,22H,1-4,7-15H2,show more
    Synonyms
    N-[2-[2-[2-[(3-Maleimidopropionyl)amino]ethoxy]ethoxy]ethyl]biotinamide | Biotin-PEG2-NH-Mal | B3174 | SCHEMBL9893190...
  8. (+)-Biotin-PEG₃-amine - ≥95% CAS No(359860-27-8) by Aladdin Scientific
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    (+)-Biotin-PEG₃-amine
    CAS: 359860-27-8 EC Number: 884-847-9 Formula: C18H34N4SO5 Molecular Weight: 418.55
    Solid Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools. ≥95%
    In Stock Item #: B122227
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-(2-aminoethoxy)ethoxy]ethoxy]ethyl]pentanamide
    SMILES
    C1C2C(C(S1)CCCCC(=O)NCCOCCOCCOCCN)NC(=O)N2
    InChIKey
    KIJSBKNJFAUJFV-ZOBUZTSGSA-N
    InChI
    1S/C18H34N4O5S/c19-5-7-25-9-11-27-12-10-26-8-6-20-16(23)4-2-1-3-15-17-14(13-28-15)21-18(24)22-17/h14-15,17H,1-13,19H2,(H,20,23)(H2,21,22,24)/t14-,15-,show more
    Synonyms
    (+)-Biotinyl3,6,9-trioxaundecanediamine | AKOS005166837 | Biotin-PEG3-CH2CH2NH2 | (+)-Biotin-PEG3-CH2CH2NH2 | PD07847...
  9. (+)-Biotin-PEG₄-amine - 95%, used for , CAS No. 663171-32-2, by Aladdin Scientific
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    (+)-Biotin-PEG₄-amine
    CAS: 663171-32-2 Formula: C20H38N4SO6 Molecular Weight: 462.60
    ≥95%
    In Stock Item #: B122228
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-(2-aminoethoxy)ethoxy]ethoxy]ethoxy]ethyl]pentanamide
    SMILES
    C1C2C(C(S1)CCCCC(=O)NCCOCCOCCOCCOCCN)NC(=O)N2
    InChIKey
    GVGZXRGPGZMYTB-LNLFQRSKSA-N
    InChI
    1S/C20H38N4O6S/c21-5-7-27-9-11-29-13-14-30-12-10-28-8-6-22-18(25)4-2-1-3-17-19-16(15-31-17)23-20(26)24-19/h16-17,19H,1-15,21H2,(H,22,25)(H2,23,24,26)/show more
    Synonyms
    (3aS,4S,6aR)-N-(14-Amino-3,6,9,12-tetraoxatetradec-1-yl)hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide | N-[...
  10. Biotin-PEG11-Amine
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    Biotin-PEG11-Amine
    CAS: 1418022-42-0 Formula: C34H66N4O13S Molecular Weight: 770.98
    ≥93%(HPLC)
    In Stock Item #: B405290
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-aminoethoxy)ethoxy]ethoxy]ethoxy]ethoxyshow more
    SMILES
    C1C2C(C(S1)CCCCC(=O)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCN)NC(=O)N2
    InChIKey
    SHPHAOSBFNWGAC-PHDGFQFKSA-N
    InChI
    1S/C34H66N4O13S/c35-5-7-41-9-11-43-13-15-45-17-19-47-21-23-49-25-27-51-28-26-50-24-22-48-20-18-46-16-14-44-12-10-42-8-6-36-32(39)4-2-1-3-31-33-30(29-5show more
    Synonyms
    N-Biotinyl-3,6,9,12,15,18,21,24,27,30,33-undecaoxapentatriacontane-1,35-diamine | N-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-...
  11. N-GlcNAc-Biotin
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    N-GlcNAc-Biotin
    CAS: 1272755-69-7 PubChem CID: 44629936 Formula: C₁₈H₃₀N₄O₇S Molecular Weight: 446.52
    Out of Stock Item #: N404501
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    Technical Identifiers
    IUPAC Name
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[(2R,3S,4R,6S)-4,5-dihydroxy-6-(hydroxymethyl)-3-(2-oxopropyl)oxan-2-yl]penshow more
    SMILES
    CC(=O)CC1C(C(C(OC1NC(=O)CCCCC2C3C(CS2)NC(=O)N3)CO)O)O
    InChIKey
    FSUXTBPGXKXZDJ-NWGRMCGKSA-N
    InChI
    1S/C19H31N3O7S/c1-9(24)6-10-16(26)17(27)12(7-23)29-18(10)21-14(25)5-3-2-4-13-15-11(8-30-13)20-19(28)22-15/h10-13,15-18,23,26-27H,2-8H2,1H3,(H,21,25)(Hshow more
    Synonyms
    5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[(2R,3S,4R,6S)-4,5-dihydroxy-6-(hydroxymet...
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R&D Use Only Products supplied for research and development use
Biotinylation reagents are used to label biomolecules for capture, detection, enrichment, and interaction workflows. Selection often depends on reactive group, spacer length, cleavability, solubility, target biomolecule, and compatibility with the labeling conditions and downstream avidin-based workflow. This category is useful when building capture or pull-down strategies that need predictable biotin installation and downstream handling. It complements crosslinkers, PEG linkers, and protein interaction workflows.
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