Immobilized Cefradine Acylase, CAS No.9014-06-6

CAS: 9014-06-6 Cat. No.: A1436587 EG-Nummer: 232-753-8

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, >50 U/g wet weight

Bioactivity

>50 U/g wet weight

Übersicht

It produces cefradine from 7-ADCA (7-amino-3-deacetoxy-cephalosporanic acid) and DHME (D-dihydrophenylglycine methyl ester).

Standard

Activity: >50 U/g wet weight

Size of immobilization support: 250~500μm (>95%)

Water content <65%

Enzyme assay: One unit (U) represents the amount of enzyme required for producing1μmol cefradine per min in 0.1M K-phosphatebuffer containing 20mM 7-ADCA and (7-ami-no-3-deacetoxy-cephalosporanic acid) and 20mM DHME(D-dihy-drophenylglycine methyl ester), , pH 7.5 at 28°C.

Application 

Cefradine production from 7-ACDA and DHME

Characteristics 

·High synthesis activity of cefradiner

·Low degradation activity of ccefradine and DHME

·Fast enzyme reaction(reaction completed in 90 mins)

·High yield synthesis of cefradine (>95%) 

Reaction condition 

pH	8.0 ~ 8.3
Temperature	10℃
Solvent	Water
7-APRA：HPGM	1:1.05-1.10
Enzyme amount	7U/ml

Scheme for enzyme reaction 

Optional reaction conditions (Temperature, pH) 

Production instruction

Category	Contents
Materials	- Synthesizing enzyme:  Immobilized Cefradine Acylase (80U/g wet, 65% water content) - 7-ADCA, DHME·HCl - Water - Ammonium hydroxide (28%)
Condition	- pH: 8.0-8.2 - Temperature: 10℃ - 7-ADCA：DHME: 350mM:385mM(1:1.10) - Enzyme: 7U/ml - Reaction solution: Water
Step	1. Remove the water after washing immobilized enzyme with water more than five times. 2. Dissolve 7-ADCA(350mM) with ammonium hydroxide and water , and then add DHME·HCl (385mM). Adjust pH to 8.0 and volume to 70ml. 3. Pour 6.13g wet enzyme in conversion reactor. 4. Proceed synthetic reaction at 10℃ and 550rpm after pouring substrates. 5. Extract every 60 minutes after 1 hour. Stop the reaction if conversion rate of 7-ADCA is more than 98.5%. 6. Take the reaction solution in reactor.

Fig 3. Cefradine production from Immobilized Cefradine Acylase

Specifications

Product Name

Immobilized Cefradine Acylase, CAS No.9014-06-6

Synonyme

Cefradine acylase (Immobilized)

Note

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Spezifikationen & Reinheit

Bioactive,ActiBioPure™,High Performance,EnzymoPure™,>50 U/g wet weight

Bioaktivität

>50 U/g wet weight

CAS

9014-06-6

Enzym Kommissionsnummer

EC 3.5.1.11

Molekültyp

Enzyme

Lagerung und Versand

Konzentration

>50 U/g wet weight

Storage

Store at 2-8°C

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Definition der Einheit

One unit (U) represents the amount of enzyme required for producing1μmol cefradine per min in 0.1M K-phosphatebuffer containing 20mM 7-ADCA and (7-ami-no-3-deacetoxy-cephalosporanic acid) and 20mM DHME(D-dihy-drophenylglycine methyl ester), , pH 7.5 at 28