ANO1-IN-4 , CAS No.2098490-06-1

CAS: 2098490-06-1 Cat. No.: A1437888 Summenformel: C₁₉H₁₉BrF₂N₂O₂S Molekulargewicht: 457.33

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Übersicht

ANO1-IN-4 (Compound 10bm) is a reversible inhibitor for calcium-activated chloride channel transmembrane protein 16A ( TMEM16A , also known as ANO1 ) with an IC 50 of 0.030 µM. ANO1-IN-4 exhibits good metabolic stability in rat liver microsomes. ANO1-IN-4 inhibits spontaneous contraction in mouse isolated ileum.

Spezifikationen

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Aktionsart

INHIBITOR

Namen und Kennungen

Molekulargewicht

457.33

Application Protocols

Item-specific (from Product Data)

• No validated application protocols or recommended dilutions are provided for this item.

General protocols for small-molecule inhibitors (literature/general)

• Stock preparation: Prepare a concentrated DMSO stock (e.g., 10–50 mM) under dry, ambient conditions; vortex to dissolve. Filter if particulate persists and the compound is filter-stable.
• Cell-based assays: Dilute stock into pre-warmed culture medium to the desired final concentration, keeping the DMSO constant across wells (typically ≤0.1–0.5% v/v). Include vehicle controls and perform a 10-point half-log dose–response for IC50 determination.
• Electrophysiology: Preincubate cells expressing ANO1 with inhibitor for 5–15 minutes; record whole-cell currents under defined Ca2+ activation. Use series resistance compensation and control for rundown.
• Data quality: Include technical triplicates, independent biological replicates, and verify compound stability over the assay timeframe.

These are general suggestions and not validated for A1437888. Consult the CoA and SDS before use.

Biological Roles

Context (literature/general)

• ANO1/TMEM16A is a calcium-activated chloride channel expressed in diverse tissues, regulating chloride secretion, smooth muscle contraction, sensory transduction, and epithelial fluid homeostasis.
• Modulation of ANO1 function influences membrane excitability, epithelial transport, and signaling pathways linked to Ca2+ dynamics. Inhibitors are used to dissect the physiological role of chloride currents and to map downstream effects on cellular phenotypes.

Research applications of ANO1 inhibitors (general)

• Target validation: Determine the contribution of ANO1 currents in model systems using pharmacological block alongside genetic perturbations (siRNA/CRISPR).
• Pathway mapping: Examine cross-talk with Ca2+ signaling, Rho/ROCK pathways, and kinases that affect ANO1 trafficking/activity.
• Disease-model research: Probe the involvement of ANO1 in airway, gastrointestinal, sensory, or secretory epithelia using ex vivo tissues and engineered cell lines. These are research uses only.

Note on specificity

• Small-molecule inhibitors can exhibit off-target effects, particularly among other TMEM16 isoforms and unrelated channels/transporters. Include counter-screens and chemically distinct inhibitors to strengthen conclusions.

Item-specific detail

• No potency, selectivity, or biological activity data are provided for this item; consult the CoA/Spec Sheet and primary literature for ANO1-IN-4.

Buffer Applications

Not typically applicable.

• ANO1-IN-4 is a bioactive small molecule used as a research inhibitor, not a buffering agent. It does not define pH ranges or buffer capacity.

Practical note (general)

• When preparing biological assay buffers that include small-molecule inhibitors, maintain consistent vehicle content (e.g., DMSO 0.1–0.5% v/v), verify pH after addition, and filter-sterilize if compatible. Use standard buffers (HEPES, PBS) suited to your assay conditions.

Green Alternatives

Applicability

• This product is a bioactive small-molecule tool compound rather than a process solvent or bulk reagent. Green chemistry considerations therefore focus on solvent choice and waste minimization during handling and assays, rather than replacing the compound itself.

Greener handling strategies (general)

• Prefer aqueous-compatible assay formats to reduce DMSO percentage (≤0.1–0.2% v/v where assay allows).
• Use microplate-based assays to minimize compound and solvent consumption.
• Choose lower-toxicity, biodegradable detergents (e.g., polysorbates) when surfactants are needed for solubilization.
• Implement closed, low-evaporation containers and cold storage to extend shelf-life and reduce waste.

Comparison of common vehicles (general)

• DMSO: High solvency; low volatility; minimize use and ensure proper waste streams.
• Ethanol: More benign profile but lower solvency for many lipophilic inhibitors.
• Aqueous cyclodextrin formulations: Can enhance apparent solubility and reduce organic solvent load; verify target engagement and compound stability.

No greener “substitute compound” is suggested because biological target engagement is structure-specific.

Pharmaceutical Uses

Scope

• This catalog item is offered for research use only. No clinical or therapeutic use is claimed or implied.

Formulation context (general)

• In drug discovery workflows, ANO1 inhibitors may be formulated into DMSO stocks for in vitro assays and, in preclinical research settings, into suitable vehicles for pharmacology studies. Such activities are outside the scope of this catalog listing and must follow institutional and regulatory guidelines.

Standards/compendia

• There is no pharmacopeial status provided for this item. Do not use for extemporaneous compounding or as an excipient.

Item-specific information

• Excipient roles, dosage forms, and GMP status: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Calculated/literature values (not item-specific)

• Because no structure is provided, computed properties (logP, pKa, TPSA) and literature constants (mp, bp, density, refractive index) cannot be assigned for this catalog item. Consult the CoA or request technical data.

General expectations for small-molecule inhibitors (literature/general)

• Many ANO1 tool inhibitors are neutral or weakly basic heteroaromatics with moderate lipophilicity (typical clogP ~2–5) and are commonly formulated as DMSO stocks. However, these are class trends and not specifications for this item.
• Solubility notes (general): Bioactive small molecules are often soluble in polar aprotic solvents (DMSO, DMF, NMP) and variably soluble in aqueous buffers depending on ionization state; always verify with item-specific solubility data.

Do not use the above trends as specifications. For definitive physical data, refer to the CoA/Spec Sheet for A1437888.

Quality and Grades

Item-specific (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grades (general guidance)

• Research grade: Common for screening compounds and tool inhibitors; typically accompanied by HPLC purity on CoA. “Assay” refers to chromatographic purity, not potency.
• HPLC/LC–MS trace: Low-level impurities, residual solvents, and identity confirmation are often documented. If your application is biophysical or cellular, ensure purity is compatible with assay sensitivity (often ≥95% by HPLC for screening).
• Salt form vs free base/acid: Impacts solubility and handling; verify the exact form on the CoA.

What to check on receipt

• Verify identity (CAS 2098490-06-1) and batch number against CoA.
• Review purity method (UV vs ELSD vs MS), retention time, and solvent system for HPLC.
• Confirm water content and residual solvents if relevant to your assay or storage; if not listed, request additional data.

Stabilizers/additives

• Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

Item-specific (from Product Data)

• Manufacturer Applications: Not provided.

Use domain (literature/general)

• ANO1-IN-4 is commonly cited as a research tool compound for modulating the calcium-activated chloride channel ANO1/TMEM16A in biochemical, electrophysiological, and cellular assays. Typical applications include target validation, pathway interrogation, and phenotypic screening where chloride conductance is implicated.

Assay considerations (general)

• Electrophysiology: Whole-cell patch clamp or Ussing chamber assays to quantify chloride currents; control for vehicle (DMSO) and determine IC50 using concentration–response curves.
• Fluorescent halide-sensitive dyes: YFP/I- quenching assays in ANO1-expressing cells to profile inhibition kinetics; optimize dye loading and temperature.
• Orthogonal assays: Use multiple readouts (current, fluorescence, and phenotype) to mitigate assay artifacts.

Selectivity profiling (general)

• Evaluate activity against related TMEM16 family members and other ion channels/transporters to establish selectivity windows; consult literature or contract screening panels.

Note: The above describes typical uses of ANO1 inhibitors in research and is not item-specific performance data for A1437888.

Reaction Conditions

Not typically applicable.

• As a finished small-molecule inhibitor, ANO1-IN-4 is generally used in biological assays rather than as a reactant. No synthetic reaction condition guidance is associated with this catalog item.

Assay preparation (general)

• Prepare serial dilutions from a verified-concentration DMSO stock into assay buffer immediately before use to minimize adsorption and degradation.
• Equilibrate compounds and plates to room temperature before dispensing to improve accuracy with DMSO solutions.
• For kinetic studies, preincubate compound with cells or membranes as appropriate and record time-dependent effects.

Safety and Handling

Item-specific (from Product Data)

• Storage conditions: Store at -20°C.
• Shipping: Ice chest + ice pads.
• GHS signal word, H-statements, classification, pictograms: Not specified for this item; refer to SDS.

General laboratory safety (literature/general)

• Handle small-molecule research inhibitors in a chemical fume hood. Avoid inhalation and skin/eye contact.
• PPE: lab coat, safety glasses, appropriate gloves (e.g., nitrile). Use additional protection when weighing powders or making concentrated DMSO stocks.
• Incompatibilities (general): Strong oxidizers and strong acids/bases may be incompatible depending on functional groups; consult the SDS before combining with reagents.
• First aid (overview): If skin contact occurs, wash with soap and water; for eye exposure, rinse with water for at least 15 minutes and seek medical attention; if inhaled, move to fresh air; if ingested, seek medical attention. Always follow the SDS.
• Waste: Dispose via organic hazardous waste stream in accordance with institutional and local regulations.

Authoritative safety information resides in the Safety Data Sheet (SDS). Always consult the SDS for A1437888 prior to use.

Solvent Selection

Item-specific (from Product Data)

• No solvent/solubility specifications provided; refer to CoA/Spec Sheet.

General guidance for small-molecule inhibitors (literature/general)

• Stock solutions: DMSO is the most common solvent for preparing 10–50 mM master stocks, due to broad solvating power and assay compatibility at ≤0.1–0.5% v/v in final wells.
• Alternative solvents: DMF or NMP can be used for poorly DMSO-soluble compounds; dilute into aqueous buffers immediately before use to minimize precipitation.
• Aqueous work: Solubility in PBS/HEPES depends on ionization; co-solvent approaches (DMSO ≤0.5% plus surfactant like 0.01% Tween-20) can help maintain homogeneity in cell-based assays.
• Volatile organics: For analytical sample prep, MeCN or MeOH may be suitable for LC–MS vials, provided compound stability is verified.

Comparison (general)

• DMSO: Highest solvency, non-volatile, hygroscopic.
• DMF/NMP: Strong solvency; higher boiling and potential toxicity; ensure thorough removal if needed.
• MeCN/MeOH: Good for LC–MS; limited solubility for more lipophilic scaffolds.

Always confirm item-specific solubility and stability from the CoA or by small-scale testing.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage: Store at -20°C.
• Shipping: Ice chest + ice pads.

General best practices (literature/general)

• Keep container tightly closed, protected from light and moisture. Allow vial to equilibrate to room temperature in a desiccator before opening to minimize condensation.
• Reconstitution: If the item is provided as a solid, prepare a DMSO stock at a convenient concentration (e.g., 10–50 mM), based on confirmed solubility. If provided in solution, verify concentration by UV or weight-by-difference where applicable.
• Aliquoting: Dispense single-use aliquots to avoid repeated freeze–thaw. Store aliquots at -20°C or below. Avoid frost-free freezers due to temperature cycling.
• Stability checks: Periodically assess by HPLC/LC–MS for degradation. Discard solutions showing precipitation, discoloration, or new peaks.

Any specific shelf-life, solubility, or stabilizer information is not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

Item-specific (from Product Data)

• Product name: ANO1-IN-4 (SKU: A1437888)
• CAS: 2098490-06-1
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.

Context (literature/general)

• ANO1-IN-4 is a literature name typically used for a small-molecule inhibitor targeting the calcium-activated chloride channel ANO1 (also known as TMEM16A). Without a provided structure, specific functional-group analysis cannot be given for this catalog item.

Structure description

• 2D/functional features: Not specified for this item; refer to CoA/Spec Sheet.
• Stereochemistry: Not specified for this item; refer to CoA/Spec Sheet.

Identity confirmation guidance (general)

• Recommended methods: HRMS, 1H/13C NMR, HPLC/UPLC purity assessment, and, if chiral, optical rotation or chiral HPLC. Compare to the CoA reference and published spectral data when available.

Synthetic Utility

Not a building block reagent.

• ANO1-IN-4 is a designated bioactive tool compound rather than a general synthetic intermediate. It is not typically used to construct other molecules in multistep synthesis.

General note (literature/general)

• If derivatization is desired for probe development (e.g., biotinylation, fluorescent tagging), identify modifiable positions from the disclosed structure in primary literature, and use linkers that preserve activity. Without the structure for this item, no site-specific guidance can be provided.

Target Specificity

Item-specific (from Product Data)

• No target, potency, or selectivity data provided for this item.

Literature/general context

• Compounds named “ANO1-IN-#” are commonly described as inhibitors of ANO1 (TMEM16A), a calcium-activated chloride channel. However, without item-specific data, no definitive claims can be made regarding the selectivity profile, IC50, or off-targets for A1437888.

Recommendation

• Verify target engagement in your system using orthogonal assays and, where possible, compare with well-characterized reference inhibitors and genetic knockdown/knockout controls.

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2098490-06-1, the molecular formula is C19H19BrF2N2O2S, and the molecular weight is 457.33 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.