The cell wall of mycobacteria contains a large amount of lipids that surround the peptidoglycan layer, so mycobacteria are generally difficult to stain. Traditional staining methods require heating and prolonged staining time to promote dyeing. Once mycolic acid in mycobacteria combines with the dye, it is difficult to be decolorized by acid decolorizing solution, hence the name acid-fast staining. One of the most representative methods is the Ziehl-Neelsen staining method for Mycobacterium tuberculosis, which is the heat staining method recommended by the WHO.
Aladdin Acid-fast Stain Solution (Kinyoun Cold Staining Method) is a cold stain solution that does not require heating and is relatively safer than acid-fast heat stain solutions. Its staining principle is that at room temperature, mycolic acid combines with fuchsin to form a complex. After counterstaining with methylene blue, mycobacteria remain red, while other bacteria and substances in the background turn blue. This staining method results in a darker color than the modified Kinyoun cold staining method and is more suitable for routine acid-fast staining. The Kinyoun cold staining method is recommended as the standard acid-fast staining method for laboratories or clinical settings.
Product Components and Storage Conditions:
A1510404
| Component
| 3×50mL
| 3×100mL
| Storage
|
A1510404A
| Kinyoun Fuchsin Stain Solution
| 50mL
| 100mL
| RT. Store in the dark.
|
A1510404B
| Kinyoun Decolorizing Solution
| 50mL
| 100mL
| RT
|
A1510404C
| Methylene Blue Stain Solution
| 50mL
| 100mL
| RT. Store in the dark. |
Self-prepared Materials:
1. Inoculating loop
2. Glass slide
3. Distilled water
4. Microscope
Operating Procedures (For Reference Only):
1. Pick the test sample with an inoculating loop, spread it evenly on the glass slide, and air-dry naturally.
2. Add a drop of Kinyoun Fuchsin Stain Solution and stain for 5–10 minutes.
3. No heating required. Rinse the slide with distilled water.
4. Decolorize with Kinyoun Decolorizing Solution until no red color is visible.
5. Rinse with distilled water.
6. Stain with Methylene Blue Stain Solution for 30–60 seconds.
7. Rinse with distilled water.
8. Blot the water gently and air-dry naturally.
9. Examine under the oil immersion lens of the microscope.
Precautions:
1. Tighten the bottle caps immediately after each use to prevent reagent volatilization and contamination.
2. All the above reagents are irritating to the human body; please take appropriate protective measures.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
4. This product is for research use only. Any other applications are strictly prohibited.
ZJ26F0332975