Antacid Stain Solution (Modified Kinyoun Cold Staining Method) - BioReagent,Suitable for microbiology,Biological Stain,for microscopy

Cat. No.: A1510398

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain, for Microscopy

Synonyms

Acid-fast Bacilli Stain Solution | Weak Acid-fast Stain Solution | Nocardia Stain Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Microbial staining

Overview

The cell wall of mycobacteria contains a large amount of lipids that encase the peptidoglycan layer, which is why mycobacteria are generally difficult to stain. Traditional staining methods require heating and extended staining time to facilitate dyeing. Once mycolic acid in mycobacteria binds to the dye, it is resistant to decolorization by acidic decolorizing solution — hence the name acid-fast staining. The Acid-fast Stain Solution (Modified Kinyoun Cold Staining Method) is a cold-type stain that does not require heating, making it relatively safer than heat-based acid-fast stain solutions. Its staining principle is as follows: at room temperature, mycolic acid combines with fuchsin to form a stable complex. After counterstaining with methylene blue, mycobacteria remain bright red, while other bacteria and background substances are stained blue.

Product Components and Storage Conditions:

A1510398	Component	3×50mL	3×100mL	Storage
A1510398A	Modified Kinyoun Fuchsin Stain Solution	50mL	100mL	RT. Store in the dark.
A1510398B	Kinyoun Decolorizing Solution	50mL	100mL	RT
A1510398C	Methylene Blue Stain Solution	50mL	100mL	RT. Store in the dark.

Operating Procedures (For Reference Only):

1. Pick the test sample with an inoculating loop, spread it evenly on a glass slide, and air-dry naturally.

2. Add a drop of Modified Kinyoun Fuchsin Stain Solution and perform staining.

3. Heating is mandatory. Rinse the slide with distilled water.

4. Decolorize the slide with Kinyoun Decolorizing Solution.

5. Rinse with distilled water.

6. Stain the slide with Methylene Blue Stain Solution.

7. Rinse with distilled water.

8. Blot the water gently and air-dry naturally.

9. Examine the slide under an oil immersion lens.

Staining Results:

Acid-fast or weakly acid-fast bacteria: Red

Non-acid-fast bacteria, cells, and background: Light blue

Precautions:

1. Tighten the reagent bottle caps immediately after each use to prevent reagent volatilization and contamination.

2. All the above reagents are irritating to the human body; please take appropriate protective measures.

3. For your safety and health, wear a lab coat and disposable gloves during operation.

4. This product is for research use only. Any other applications are strictly prohibited.

Specifications

Synonyms

Acid-fast Bacilli Stain Solution | Weak Acid-fast Stain Solution | Nocardia Stain Solution

Specifications & Purity

BioReagent, Suitable for microbiology, Biological Stain, for microscopy

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy, Suitable for microbiology

Chemical and Physical Properties

• Sensitivity: Light-sensitive