Apotransferrin, Rat Plasma

CAS: 11096-37-0 Cat. No.: np001124

GRADE & PURITY Native, Lyophilized powder, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration (see COA)

Accession #

P12346

Übersicht

ApoTransferrin is the iron-free form of transferrin, serves as the primary plasma transporter of essential Fe³⁺. Apotransferrin limits microbial growth by sequestering free iron, and can be an important supplement for serum free media.

Role of protein in biology

Apotransferrin, the iron-free form of the glycoprotein transferrin, serves as a critical regulator of systemic iron homeostasis by binding and transporting ferric ions (Fe³⁺) through plasma via a pH-dependent mechanism. It exhibits a structure with two iron-binding sites. Beyond its primary role in iron distribution, apotransferrin modulates immune function by sequestering free iron to limit microbial growth and directly suppresses proinflammatory cytokine production in autoimmune conditions like type 1 diabetes. Clinically, congenital atransferrinemia-caused by TF gene mutations-manifests as severe microcytic anemia coupled with tissue iron overload, requiring lifelong apotransferrin replacement therapy.

In biotechnology, ultra-purified apotransferrin (<0.01% iron content, 1.25 μg/mg iron-binding capacity) serves as an essential cell culture supplement, facilitating iron uptake through transferrin receptor-mediated endocytosis while preventing oxidative stress. Recent applications exploit its targeting potential in nanoparticle drug delivery systems for cancer therapeutics and as an antimicrobial adjuvant to enhance antibiotic efficacy against Gram-negative pathogens. The protein's solubility (10 mg/ml in aqueous solutions) and stability under physiological conditions enable its use in diagnostic assays and bioreactor media for biopharmaceutical production.

Specifications

Product Name

Apotransferrin, Rat Plasma

Note

Lyophilized powder, Native

Spezifikationen & Reinheit

Native, Lyophilized powder, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration (see COA)

Biochemische und physiologische Mechanismen

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage a

Beitritt #

P12346

Voraussichtliches Molekulargewicht

80 kDa

CAS

11096-37-0

Molekültyp

Protein

Product Line

biological_products

Produkteigenschaften

ALogP

-2.5

Lagerung und Versand

Form

lyophilized from 20 mM NaH2PO4, pH 7.4, 150 mM NaCl,

Konzentration

Pre-lyophilization Protein Concentration (see COA)

Rekonstitution

After centrifugation, we suggest addition of de-ionized water to the original volume (See COA), followed by gentle mixing to ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20℃ long term (>1 year). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.