GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Rabbit anti Human ARPC4 Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 0.01-2µg/mL IHC: 5-20µg/mL
IF/ICC: 2.3µg/mL Protein Function Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Specifications
Product Name
ARPC4 Antibody - Primärer Antikörper, specific to ARPC4, Rabbit IgG
Synonyme
Actin related protein 2/3 complex subunit 4 20 kD antibody | Actin related protein 2/3 complex subunit 4 20kDa antibody | Actin related protein 2/3 complex, 20-KD subunit antibody | Actin-related protein 2/3 complex subunit 4 antibody | ARC20 antibody | A
Spezifikationen & Reinheit
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Wirtsspezies
Kaninchen
Spezifität
ARPC4
Immunogen
Recombinant ARPC4 expressed in E.coli (AA 3-165)
Positive Kontrolle
WB: HeLa, Jurkat, HEK293, THP-1, NIH/3T3 and C6 cell lysates. IHC: Human liver tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Antigen affinity purified + Protein A purified
Konzentration
See COA
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
ARPC4 Antibody (Ab089682) - Western Blot All lanes: ARPC4 Antibody (Ab089682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 5.0 s
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Control: Used PBS instead of primary antibody Second Ab: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IF/ICC IF analysis of ARPC4 Antibody (Ab089682) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARPC4 Antibody (Ab089682) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
2.3µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Wir verwenden Cookies, um die ordnungsgemäße Funktion der Website sicherzustellen und, soweit zulässig, Ihr Nutzererlebnis zu verbessern. Sie können Ihre Einstellungen jederzeit unter „Einstellungen“ verwalten. Weitere Informationen finden Sie in unserer Cookie-Richtlinie.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0708816