ARRDC1 Antibody - Primärer Antikörper, specific to ARRDC1, Rabbit IgG

Cat. No.: Ab089692

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human ARRDC1 Antibody, Polyclonal, could be used for WB, IF/ICC and so on.

Specifications

Product Name

ARRDC1 Antibody - Primärer Antikörper, specific to ARRDC1, Rabbit IgG

Synonyme

ARRD1_HUMAN antibody | ARRDC 1 antibody | ARRDC1 antibody | Arrestin domain containing 1 antibody | Arrestin domain containing protein 1 antibody | Arrestin domain-containing protein 1 antibody | MGC40555 antibody | OTTHUMP00000022707 antibody | OTTHUMP00

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates.Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles. More generally, plays a role in

Wirtsspezies

Kaninchen

Spezifität

ARRDC1

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARRDC1.

Positive Kontrolle

WB:HeLa, NIH/3T3 and C6 cell lysates. IF/ICC: MDA-MB-231 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: ARRDC1 Antibody (Ab089692)

ARRDC1 Antibody (Ab089692) - Western Blot
All lanes: ARRDC1 Antibody (Ab089692) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
Exposure time: 86.8 s

IF/ICC: ARRDC1 Antibody (Ab089692)

ARRDC1 Antibody (Ab089692) - IF/ICC
Immunofluorescent analysis of ARRDC1 staining in MDA-MB-231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ARRDC1 Antibody (Ab089692) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/100-1/500 ;

Zugehörige Ziele (menschlich)

ARRDC1 Tbio Arrestin domain-containing protein 1 (0 Activities)

Discover Target: ARRDC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)