ARRDC3 Antibody - Primärer Antikörper, specific to ARRDC3, Rabbit IgG

Cat. No.: Ab089693

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Affe, Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human ARRDC3 Antibody, Polyclonal, could be used for WB, IHC and so on.

Specifications

Product Name

ARRDC3 Antibody - Primärer Antikörper, specific to ARRDC3, Rabbit IgG

Synonyme

ARRDC 3 antibody | Arrestin domain containing 3 antibody | Arrestin domain containing protein 3antibody | KIAA1376antibody | TBP-2-like inducible membrane protein antibody | Thioredoxin binding protein 2 like inducible membraneantibody | TLIMPantibody | A

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed:20559325, PubMed:21982743, PubMed:23208550). Plays a role in NEDD4-mediated ubiquitination and endo

Wirtsspezies

Kaninchen

Spezifität

ARRDC3

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ARRDC3.

Positive Kontrolle

WB: MCF7 cell lysate. IHC: Human brain tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - Western Blot
All lanes: ARRDC3 Antibody (Ab089693) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 2.9 s

IHC: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - IHC
Immunohistochemical analysis of ARRDC3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ARRDC3 Antibody (Ab089693) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - IF/ICC
IF analysis of ARRDC3 Antibody (Ab089693) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARRDC3 Antibody (Ab089693) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Anwendung

• Anwendung: Dilution info
• IF/ICC:

  1/200;

• WB:

  1/500-1/1000 ;

• IHC:

  1/50-1/100 ;

Zugehörige Ziele (menschlich)

ARRDC3 Tbio Arrestin domain-containing protein 3 (0 Activities)

Discover Target: ARRDC3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)