Bacillus cereus Chromogenic Medium - BioReagent

Cat. No.: B1528764

GRADE & PURITY BioReagent

Storage

Store at 2-8°C

Shipped In

Wet ice

Übersicht

For rapid detection of Bacillus cereus; Bacillus cereus appears blue-green

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Store at 2-8°C

Verschickt in

Wet ice

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

Only product‑validated protocols should be listed here; none are provided in the Product Data.

• Tested applications, recommended incubation temperatures/times, colony color charts, and control strain panels: Not specified for this item; refer to the product insert/CoA.

For general workflow guidance (non‑validated), see Reaction Conditions and Reaction & Applications. Always follow your laboratory’s SOPs and method‑specific instructions.

Biological Roles

This section provides biological context for the target organisms detected by the medium (general literature information; not product‑specific performance claims).

• Bacillus cereus group: Comprises closely related, spore‑forming, Gram‑positive rods (e.g., B. cereus sensu stricto, B. thuringiensis, B. anthracis, B. weihenstephanensis, among others) sharing high genomic similarity but differing in plasmid content and phenotypic traits.
• Environmental ecology: Ubiquitous in soil, dust, and plant material; spores survive harsh conditions and can contaminate raw materials and processing environments.
• Metabolic/enzymatic traits leveraged by chromogenic media: Many B. cereus group members express phosphatidylinositol‑specific phospholipase C and exhibit lecithinase activity; they are typically mannitol non‑fermenters and may display characteristic hemolysins (literature). Chromogenic substrates are selected to reveal one or more of these activities as distinctive colony colors/halos.
• Growth preferences (literature): Aerobic or facultatively anaerobic; robust growth on nutrient‑rich, neutral‑pH media at 30–37°C, with psychrotolerant strains growing at lower temperatures. Spore germination and outgrowth are influenced by medium composition and incubation parameters.
• Confirmatory workflows: Because the group includes species with overlapping phenotypes, presumptive colonies from chromogenic media are often subjected to confirmatory assays (biochemical panels, MALDI‑TOF MS, PCR for species/virulence markers) to refine identification in research/testing contexts.

Buffer Applications

Not applicable. This product is a complete microbiological culture medium rather than a buffer system. For pH control considerations, see Physical Properties (typical pH ranges, literature) and Reaction & Applications (incubation parameters).

Green Alternatives

While culture media are not typically evaluated under solvent‑green chemistry metrics, sustainability considerations still apply.

• Format selection: Dehydrated media minimize shipping mass/volume and packaging, reducing transport footprint; ready‑to‑use plates reduce in‑lab energy/water consumption by avoiding autoclaving and pouring. Choose based on your facility’s resource profile.
• Energy efficiency: Batch‑prepare media (if dehydrated) to maximize autoclave load efficiency. Avoid overheating, which wastes energy and can degrade labile chromogenic/ selective components.
• Waste reduction: Plan plate counts to minimize expired/unused plates. Use re‑sealable secondary containment to maintain humidity and extend in‑use shelf life per label guidance.
• Safer constituents: Some selective agents and chromogens may have environmental persistence. Where method‑permissible, consider media formulations documented to use less hazardous inhibitors (literature). For regulated methods, changes may not be acceptable.
• Disposal: Autoclave inoculated media and dispose as biohazard per institutional policy to prevent environmental release of viable organisms. Segregate plastic ware for appropriate waste streams.

Item‑specific environmental certifications, recycled content, or take‑back programs: Not specified for this item; refer to product literature or contact Aladdin Scientific Support.

Pharmaceutical Uses

No therapeutic or clinical claims are made. For research/industrial laboratory use only.

Relevant non‑clinical, non‑diagnostic roles (general, literature):

• Environmental monitoring and hygiene assessments: Used in pharmaceutical, cosmetic, and food production facilities for research‑grade monitoring of spore‑forming contaminants in controlled areas, equipment surfaces, and utilities (e.g., compressed air, non‑sterile water) where permitted by internal SOPs.
• Method development and suitability testing: Evaluating recovery of Bacillus cereus group in product‑specific matrices during development of in‑house tests, including selection of enrichment steps and neutralizers for antimicrobial residues.
• Training and proficiency: Demonstrating selective/differential plating principles in QC training labs.

Compliance note:

• If media are to be used in regulated QC testing (e.g., per pharmacopeial or ISO standards), users must ensure the medium and supplier documentation meet the required performance and traceability criteria. Item‑specific certifications and validations are Not specified for this item; refer to CoA/Spec Sheet and your quality unit.

Physical Properties

Item‑specific physico‑chemical specifications are not provided in the Product Data for this mixture.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• pH (working medium): Typical chromogenic B. cereus agars are buffered near neutral (e.g., pH ~7.1–7.3 at 25°C; literature). Exact value for this item: Not specified; refer to CoA/Spec Sheet.
• Form: May be supplied as dehydrated medium or ready‑to‑use plates/bottles (literature possibilities). Exact form for this item: Not specified; refer to label/CoA.
• Solubility: Dehydrated media are formulated to disperse/dissolve in purified water prior to sterilization (literature). Item‑specific instructions: Not specified; refer to product insert.
• Melting/gelation: Agar‑containing media typically liquefy near 85–100°C and solidify near 35–45°C (literature). Item‑specific gel strength/temperatures: Not specified; refer to CoA/Spec Sheet.
• Density, refractive index, logP, boiling point: Not applicable or not defined for complex culture media; Not specified for this item.

Practical notes (general, literature):

• If supplied dehydrated, mix thoroughly to a uniform suspension before heating/sterilization to avoid localized caramelization or precipitation.
• Avoid overheating to preserve labile chromogenic substrates and selective agents.
• Final medium clarity and color will depend on the specific chromogens and indicators present; consult the product insert for the expected plate color and colony chromogenic responses.

Quality and Grades

• Item grade: BioReagent (as provided). This designation indicates suitability for laboratory research, including microbiological applications, but does not imply clinical/diagnostic use or compliance with pharmacopeial specifications.

What “BioReagent” typically entails (general):

• Raw material quality appropriate for biological research.
• Internal QC to support consistent performance in lab workflows. For culture media, this commonly includes appearance, pH (at a specified temperature), and performance (growth promotion/inhibition) with defined control strains (literature: e.g., USP/EP growth promotion tests). Exact QC tests and acceptance criteria for this item are Not specified; refer to CoA/Spec Sheet.

Stabilizers/selective agents:

• Chromogenic and selective components can be labile to heat/light. Item‑specific stabilizers or protective packaging, if any, are Not specified; refer to label/CoA.

What to check on receipt:

• Verify lot number, storage temperature (2–8°C), and format (dehydrated vs. prepared). Inspect for clumping (powder) or dehydration/condensation/contamination (plates/bottles). Any item‑specific tolerances and expected coloration are Not specified; refer to product insert.

Regulatory/use note:

• For research use only (per Product Data). Not for diagnostic or therapeutic applications. If you require media validated for regulated testing, consult applicable standards (e.g., ISO 11133, FDA BAM, or pharmacopeial monographs) and obtain the corresponding documentation.

Reaction and Applications

Primary application (research use only): selective and differential detection, enumeration, and presumptive identification of Bacillus cereus group organisms from food, environmental, water, and industrial samples.

Functional principles (literature, general):

• Chromogenic differentiation: Incorporates substrates that release a colored product upon cleavage by B. cereus–associated enzymes (e.g., phospholipase C or specific glycosidases), enabling rapid visual recognition of target colonies amid background flora.
• Selectivity: Typically includes inhibitory agents (e.g., polymyxins or other selective components) that suppress Gram‑negative bacteria and many Gram‑positives while permitting B. cereus growth.
• Differential traits: The B. cereus group is typically mannitol‑negative and lecithinase‑positive; some chromogenic media visualize these traits and/or specific enzymatic activities (literature). Exact traits visualized for this item are Not specified; refer to product insert.

Use cases (examples, non‑exhaustive):

• Food microbiology: Screening raw materials and finished products for B. cereus group contamination as part of HACCP verification (research/testing labs).
• Industrial hygiene: Surface and air monitoring in production areas where spore‑forming contaminants are a concern.
• Method development: Evaluating enrichment/dilution schemes and recovery from stressed cells using alternative pretreatments (e.g., heat shock) before plating (literature practice).

Practical tips (general):

• Streak or spread defined volumes of well‑mixed dilutions to achieve countable colony numbers. Incubate aerobically at temperatures appropriate for target recovery (often 30–37°C; literature), adhering to your method SOP.
• Confirmatory testing (e.g., hemolysis, motility, toxin gene PCR, or biochemical panels) is recommended, as colony color/morphology alone is presumptive.

Reaction Conditions

For culture media, “reaction conditions” map to preparation and incubation parameters. Item‑specific instructions are not provided here; consult the label/insert. The following are general literature guidelines for chromogenic B. cereus media:

Preparation (if dehydrated; literature):

• Disperse the specified mass of medium in purified water with thorough mixing. Heat with frequent agitation to dissolve completely. If required by the formulation, sterilize by autoclaving (commonly 121°C for 15 min); some chromogenic/selective components are heat‑labile and may be filter‑sterilized and added aseptically after cooling to ~45–50°C. Item‑specific steps: Not specified; refer to product insert.
• Pour plates in aseptic conditions; allow to solidify and dry to an appropriate surface moisture level before use.

Inoculation and incubation (literature):

• Sample processing: Use appropriate enrichment or heat‑shock protocols when targeting spores vs. vegetative cells, per method SOP.
• Inoculate by streaking for isolation or by surface/spread plating a measured volume to obtain countable CFUs.
• Incubate aerobically, typically 24–48 h at 30–37°C depending on the target strain spectrum and method. Psychrotolerant strains may require lower temperatures or extended incubation.
• Interpretation: Presumptive B. cereus colonies show formulation‑defined chromogenic colors/morphologies. Perform confirmatory tests as required.

Quality control (literature practice):

• Include positive/negative control strains to verify growth promotion and selectivity. Acceptable control outcomes for this item are Not specified; refer to CoA/Spec Sheet.

Safety and Handling

Hazard classification details are not provided in the Product Data and may vary with format (dehydrated vs. prepared medium).

• GHS classification, signal word, H‑statements, pictograms: Not specified for this item; refer to SDS.

General safety guidance (literature/best practice):

• PPE: Wear lab coat, gloves, and safety glasses when handling dry medium, hot media, or inoculated plates.
• Inhalation/skin contact: Avoid creating dust/aerosols from dehydrated medium. Wash skin thoroughly after handling.
• Thermal hazards: Liquefied media can cause burns; handle hot flasks and plates with heat‑resistant gloves.
• Incompatibilities: Store separately from strong oxidizers and acids/bases that could degrade organic components (general precaution). Keep away from microbial decontaminants/biocides that could inactivate selective agents.
• Biohazard considerations: Once inoculated, plates/broth constitute biohazardous waste. Handle within appropriate biosafety level practices (typically BSL‑2 for Bacillus cereus group; institutional risk assessment required). Dispose by autoclaving or approved biohazard treatment.
• First aid (overview; defer to SDS): If dust or splashes contact eyes, rinse with water for several minutes. If inhaled, move to fresh air. If skin contact occurs, wash with soap and water. Seek medical evaluation if irritation persists.

Always consult the product‑specific SDS and your institutional biosafety guidelines for authoritative safety information and waste disposal instructions.

Solvent Selection

Not typically applicable. This product is an aqueous microbiological medium rather than a reagent intended for chemical reaction solvent selection.

Practical notes (general, literature):

• If supplied dehydrated, purified water (e.g., ISO 3696 Grade 3 or ASTM Type II) is typically used to prepare the medium. The required volume, heating, and sterilization parameters for this item are Not specified; refer to the product insert/CoA.
• Do not substitute organic solvents; they may inhibit growth and/or inactivate chromogenic substrates/selective agents.

Storage and Reconstitution

• Storage conditions (as provided): Store at 2–8°C.
• Shipping condition: Wet ice (as provided).
• Shelf life/expiry, light sensitivity, and acceptable in‑use period after opening: Not specified for this item; refer to label/CoA.
• Reconstitution/preparation: Not specified for this item; refer to the product insert. If supplied dehydrated, preparation typically involves dissolution in purified water, controlled heating/sterilization, and aseptic pouring (literature); if supplied ready‑to‑use, equilibrate to room temperature before inoculation to minimize condensation.
• Freeze–thaw guidance: Not applicable to dehydrated powders; for prepared plates/bottles, do not freeze unless explicitly stated by the manufacturer (item‑specific instruction Not specified).
• Handling tips (general): Maintain packaging sealed to prevent desiccation or moisture uptake. Avoid extended exposure to light/heat which can degrade chromogenic/selective components. Inspect prepared media for contamination, excessive condensation, or dehydration before use.

Research use note: For research use only (as provided).

Structure and Identity

This product is a formulated chromogenic microbiological medium designed for the selective/differential detection of Bacillus cereus group organisms. It is a complex mixture (not a single defined chemical entity), thus conventional small‑molecule identifiers do not apply.

• SKU: B1528764
• Product name: Bacillus cereus Chromogenic Medium
• Category: Life Science research consumable (microbiology medium)
• Composition: Complex, multi‑component medium (peptones, buffers, salts, selective agents, chromogenic substrates, and gelling agents are commonly used in such media; literature). Exact formulation is proprietary/not provided here.
• Molecular formula: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChIKey: Not applicable for complex mixtures.

Structural/functional features (descriptive; literature):

• Includes a chromogenic substrate system that yields a visible color change in colonies expressing Bacillus cereus–characteristic enzyme activities (e.g., phospholipase C or specific glycosidases; literature), allowing rapid visual differentiation from background flora.
• Often incorporates selective agents (e.g., antibiotics, elevated NaCl, or bile salts; literature) to inhibit competing microorganisms while permitting B. cereus growth.
• Typically formulated with a buffering system to maintain near‑neutral pH conducive to Bacillus spp. growth (literature).

2D “structure” description: As a dehydrated powder or pre‑prepared agar, the medium presents as a homogeneous matrix; the “structural features” relevant to function are the embedded chromogenic substrates and selective constituents distributed throughout the gel matrix (when solidified).

Synthetic Utility

Not applicable. This product is not a chemical building block or reagent for organic synthesis; it is a microbiological culture medium for selective/differential growth of Bacillus cereus group organisms. For practical use guidance, see Reaction & Applications and Reaction Conditions.

Target Specificity

This section typically applies to antibodies or affinity reagents with defined antigen/epitope specificity and clone/isotype information. Not applicable to a culture medium. No target specificity data are provided in the Product Data.