BCIP/NBT Stock Solution, 50X - 50 X , CAS No.MFCD00466954

CAS: MFCD00466954 Cat. No.: B385716 Summenformel: NBT: C₄₀H₃₀Cl₂N₁₀O₆, BCIP: C₈H₆NO₄BrClP x C₇H₉N EG-Nummer: 229-506-1 (BCIP), 206-067-4 (NBT)}

GRADE & PURITY 50 X

Storage

Store at 2-8°C

Shipped In

Wet ice

Übersicht

5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on Western, Northern, Southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive.|Recommended for use with Crystal Mounting Medium (sc-24943).
Preparation of staining solution:•Add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride. (do not include Magnesium Chloride in the detec
BCIP/NBT Stock Solution, 50Xis a substrate for alkaline phosphatase (AP) based detection systems including western blotting

Specifications

Spezifikationen & Reinheit

50 X

Storage

Store at 2-8°C

Verschickt in

Wet ice

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Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is MFCD00466954, the molecular formula is NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.