Biotin Assay Medium (USP) - BioReagent

Cat. No.: B1528765

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For determination of biotin. Preparation method: Weigh 7.5 g of this product in 100 mL of distilled or deionized water (the volume may be scaled up or down proportionally), heat with stirring and boil until completely dissolved, dispense (stir well to ensure even distribution of insoluble matter), and set aside for use. Precautions: Avoid contact with skin and eyes during use; this product is for laboratory microbiological testing.

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

Item-specific: Manufacturer-tested applications are not provided in the Product Data.

Literature protocol outline for biotin microbiological assay (for research use only; adapt to your SOP)

1. Preparation

• Dissolve the dehydrated medium in purified water per your method or monograph. Adjust pH if specified. Sterilize by autoclaving; cool to dispensing temperature.

2. Standards and samples

• Prepare a series of biotin standards spanning the expected concentration range. Process test samples (e.g., extraction, dilution, clarification) to mitigate matrix effects while keeping biotin stable.

3. Inoculum

• Prepare a standardized inoculum of the designated Lactobacillus strain from a fresh culture according to the method’s maintenance and adaptation steps.

4. Dispensing

• Aliquot equal volumes of medium into sterile tubes or microplate wells. Add standards or samples across replicates; inoculate uniformly.

5. Incubation and readout

• Incubate at the method-defined temperature/time. Measure turbidity/OD at the specified wavelength. Include blanks and quality controls.

6. Calculations

• Construct a calibration curve (growth vs. biotin concentration). Determine sample biotin content with appropriate dilution and recovery corrections. Perform system suitability checks (linearity, r, slope, and control recoveries) each run.

Note: Exact volumes, concentrations, and conditions are Not specified for this item; consult the current USP chapter and your validated SOP.

Biological Roles

Scope

• The product itself is a culture medium. Its function in biological experiments is to enable quantification of biotin via growth response of a biotin-auxotrophic microorganism.

Relevant biology of biotin (literature/general)

• Biotin (vitamin B7/H) is an essential cofactor for carboxylases involved in central metabolism, including acetyl-CoA carboxylase, propionyl-CoA carboxylase, pyruvate carboxylase, and methylcrotonyl-CoA carboxylase. It is typically covalently attached to apocarboxylases by holocarboxylase synthetase and recycled by biotinidase.
• Microbial auxotrophy: Certain Lactobacillus species require exogenous biotin. In a biotin assay medium lacking biotin, their growth becomes strictly dependent on biotin provided by standards or test samples.

Assay biology considerations (literature)

• Response curve: Under controlled conditions, cell density after incubation correlates with log(biotin concentration) over a defined range; too high biotin may saturate response, too low yields sub-detectable growth.
• Interferences: Antimicrobials, residual sanitizers, strong chelators, and extreme pH can suppress growth independently of biotin content, biasing results. Sample preparation often includes extraction and dilution to mitigate matrix effects.
• Organism health: Maintain the test organism on prescribed maintenance media and passage regimen to preserve sensitivity and reproducibility.

Buffer Applications

Not typically applicable.

Notes

• Biotin Assay Medium (USP) is a complete nutrient medium rather than a discrete buffering reagent. While prepared media include buffering salts to stabilize pH during microbial growth, this product is not intended for general-purpose buffer preparation.
• For assays requiring precise initial pH, follow the compendial method for pH adjustment of the prepared medium rather than substituting independent buffer systems.

Green Alternatives

Context

• As a microbiological culture medium, the environmental profile is influenced by energy/water use, packaging, and waste management rather than solvent hazards.

Greener practice options (literature/good practice)

• Preparation efficiency: Batch-prepare only the volume needed per assay to reduce waste. Optimize autoclave loads to minimize energy and water usage per cycle.
• Packaging: Where available, choose larger pack sizes to reduce packaging waste per unit mass, or use returnable/recyclable containers when compatible with quality systems.
• Process water: Employ water-efficient rinsing/cleaning procedures for glassware; validate cleaning-in-place (CIP) to reduce detergent use.
• Waste minimization: Neutralize and autoclave biological waste, then segregate for appropriate disposal. Avoid adding hazardous chemicals to spent media so that waste streams remain non-hazardous where permitted by local regulations.
• Alternative formats: Ready-to-use sterile media (liquid or pre-filled vials) can eliminate autoclaving but may increase shipping mass; assess total footprint versus on-site preparation.

Trade-offs

• Dehydrated media require autoclaving (energy/water) but ship lighter than ready-to-use liquids. Selection should balance laboratory throughput, QA needs, and sustainability goals.

Pharmaceutical Uses

Scope (no therapeutic claims)

• Role: Analytical/research use medium for potency/assay of biotin in quality control environments.

Typical uses in regulated workflows (literature/general)

• QC of raw materials and finished products: Employed as part of compendial microbiological assays for measuring biotin content in vitamin formulations, dietary supplements, fortified foods, and fermentation-derived products.
• Method alignment: Laboratories operating under pharmacopeial standards (e.g., USP) may use a biotin assay medium that supports the designated test organism and assay conditions. Lot-specific verification against method suitability criteria is typically required.
• Documentation: For regulated use, retain Certificates of Analysis, preparation records, and instrument calibration logs linked to each assay batch.

Formulation/excipient context

• This medium is not an excipient and is not incorporated into drug products; it serves as an analytical tool for biotin quantification in manufacturing and release testing pipelines.

Physical Properties

Item-specific facts (from Product Data)

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage conditions: Room temperature (per Product Data). Ship: Normal.
• Molecular weight, formula: Not applicable (mixture).

General/literature characteristics of dehydrated culture media (not specifications)

• Typical form: Free-flowing powder or granules; color varies from off-white to tan depending on peptone/sugar content.
• Solubility: Freely soluble/dispersible in purified water; insoluble in nonpolar organic solvents (literature).
• pH (prepared medium): Defined by the compendial recipe after dissolution/sterilization, commonly near neutral for Lactobacillus assays; exact value depends on the monograph (literature).
• Density/bulk density: Product- and lot-dependent; not standardized across suppliers (literature).
• Refractive index, logP, bp/mp: Not applicable for heterogeneous solid mixtures.

Practical notes

• Use freshly prepared, thoroughly dissolved medium; avoid undissolved particulates which can confound turbidity measurements.
• Filter clarity is usually not required; sterilization is commonly by autoclaving per USP method (literature guidance).

Quality and Grades

Item-specific facts (from Product Data)

• Grade: BioReagent.
• Naming: “Biotin Assay Medium (USP)” indicates use in USP-style microbiological assays of biotin. Specific lot conformance to a USP monograph is Not specified for this item; refer to CoA/Spec Sheet.

What BioReagent grade implies (general)

• BioReagent-grade materials are intended for biological/biochemical workflows, with attention to performance-critical attributes such as nutrient profile consistency, low inhibitory contaminants, and reproducibility across lots. Exact impurity limits are application- and supplier-specific.

USP assay context (general)

• USP microbiological assay media are formulated to support growth of designated test organisms with the vitamin (biotin) as the limiting nutrient; growth response vs. standards enables potency determination by turbidimetric or titrimetric readouts. For QA/QC of vitamins, foods, and supplements, labs often require traceable CoA confirming medium performance with the prescribed organism and conditions.

Recommendations

• Verify lot-specific documentation (CoA) for performance verification (e.g., growth response curves, pH of prepared medium, sterility test outcome). If compendial compliance is required, ensure the batch is qualified against the current USP chapter for biotin assay.

Reaction and Applications

This item is not a chemical reactant but a culture medium tailored for biotin microbiological assays. As such, classical organic reaction families (e.g., Grignard, cross-coupling) do not apply.

Applications (literature/general)

• Microbiological assay of biotin: Supports growth of biotin-dependent lactic acid bacteria (commonly Lactobacillus plantarum or related species) where growth response (turbidity/OD at 540–650 nm) is proportional to biotin supplied by standards or test samples.
• QA/QC and analytical testing: Used in compendial and in-house methods for quantifying biotin in vitamin preparations, fortified foods, feeds, and fermentation broths.
• Method formats: Turbidimetric assays in tubes or microplates; standard-addition or calibration-curve approaches; endpoint or kinetic reads depending on lab SOP.

Practical tips (literature)

• Prepare media fresh; ensure complete dissolution. Sterilize according to the applicable monograph. Avoid extended storage of prepared medium, which can alter pH and available nutrients.
• Standardize inoculum density of the test organism to minimize variability. Include system suitability checks (e.g., slope/linearity of biotin standard curve).
• Control variables: incubation temperature, time, agitation, and initial pH strongly influence growth response. Use validated instruments for OD measurements and maintain consistent pathlengths.

Reaction Conditions

Not applicable in the context of chemical reactions.

Assay preparation conditions (literature/general guidance)

• Solvent: Purified water.
• Dissolution: Add the dehydrated medium to water with agitation until fully dissolved. Avoid localized clumping.
• Sterilization: Autoclave according to the applicable compendial method (e.g., gravity or pre-vacuum cycle). Cool to the specified dispensing temperature before inoculation.
• Incubation (assay): Inoculate with the standardized culture of the designated Lactobacillus strain. Incubate at the organism-specific temperature and time defined in the method until the growth endpoint is reached, then read turbidity/OD at the prescribed wavelength.

Important

• Exact concentrations, pH targets, inoculum levels, temperatures, and times are method-dependent and Not specified for this item; consult the current USP monograph/SOP and the product’s CoA/Spec Sheet.

Safety and Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS.
• Storage conditions: Room temperature. Keep container tightly closed and dry.

General laboratory safety (literature/good practice)

• PPE: Wear lab coat, safety glasses, and appropriate gloves when handling the dehydrated powder and prepared medium. Avoid breathing dust; handle powders in a well-ventilated area or fume hood if dust generation is possible.
• Incompatibilities: Strong oxidizers and strong acids/bases may degrade organic components of the medium. Avoid moisture uptake during storage to preserve free-flowing characteristics.
• Microbiological safety: When used with test organisms (e.g., Lactobacillus spp.), follow BSL-1 microbiological practices. Decontaminate waste by autoclaving or appropriate disinfectants.
• First aid (overview; defer to SDS): If inhaled—move to fresh air. Skin/eye contact—rinse with water for several minutes; remove contaminated clothing. If ingested—rinse mouth; seek medical attention if symptoms persist.
• Fire safety: Organic content may be combustible; use water spray, CO2, foam, or dry chemical for incipient fires. Combustion can produce CO/CO2 and nitrogenous fumes.

Always consult the product’s SDS for authoritative hazard information and emergency procedures.

Solvent Selection

Applicability to this product

• This product is a dehydrated culture medium intended to be dissolved in purified water. Organic solvent selection is not typically relevant.

General guidance (literature)

• Primary solvent: Water (purified/deionized). Use high-purity water to minimize antimicrobial residuals (e.g., chlorine, ozone) that can suppress growth of assay organisms.
• pH adjustment: If specified by the compendial method, adjust pH with dilute acid/base using sterile technique after dissolution and prior to sterilization.
• Additives: Do not add chelators, preservatives, or antimicrobials; these can suppress growth and invalidate the assay’s dynamic range.

Comparison notes (literature)

• Water vs. buffers: Some USP media include buffering salts within the formulation; external buffer systems are generally unnecessary and can alter the growth response. When a recipe calls for a defined pH, follow the monograph rather than substituting external buffers.
• Filtration vs. autoclaving: Thermolabile supplements (if any) are added aseptically post-sterilization through 0.2 µm filtration. Base medium is typically sterilized by autoclaving per method.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Room temperature. Shipped under normal conditions.

General guidance (literature; not item-specific specifications)

• Container: Keep tightly closed in a cool, dry place. Protect from moisture uptake to prevent clumping and performance drift.
• Shelf life: Refer to product label/CoA for expiration and retest dates. Do not use past expiry in regulated assays without requalification.
• Reconstitution: Dissolve the dehydrated medium in purified water following the applicable compendial recipe/SOP. Ensure complete dissolution with gentle heating/stirring if permitted.
• Sterilization: Autoclave per method. For thermolabile supplements (if used), sterile-filter and add aseptically after cooling.
• Prepared medium storage: If storage is permitted by your SOP, hold at 2–8 °C for a short defined period; avoid repeated warming/cooling. In many labs, media for microbiological assays are prepared fresh to minimize variability.

Important

• Exact preparation amounts, pH targets, sterilization cycle parameters, and allowable hold times are Not specified for this item; refer to the CoA/Spec Sheet and your governing method (e.g., USP) for definitive instructions.
• Research Use Note: For research use only.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Biotin Assay Medium (USP), SKU B1528765
• Type: Complex, multi-component culture medium for microbiological assay of biotin
• Grade/Purity: BioReagent
• Molecular formula: Not applicable (mixture)
• Molecular weight: Not applicable (mixture)
• SMILES/InChI/InChIKey: Not applicable (mixture)

General description (literature)

• Composition class: Typically a dehydrated nutrient medium formulated to be biotin-free (or biotin-limited) except for biotin supplied by test samples/standards; contains carbohydrates, nitrogen sources (amino acids/peptones), minerals, and buffer salts tailored for biotin-responsive lactic acid bacteria (e.g., Lactobacillus spp.).
• Structural features: As a heterogeneous blend of organic and inorganic ingredients, it lacks a single defined 2D structure; rather it is a composite of peptides, salts, sugars, and growth factors (minus biotin).
• Functional concept: Provides all nutrients necessary for growth of a biotin-auxotrophic test organism, such that growth (turbidity/OD) is proportional to biotin concentration supplied externally (standard/addition method per USP).

Notes

• Because this is a complex mixture, small-molecule identifiers (SMILES, InChI) and discrete structural depictions are not applicable.

Synthetic Utility

Not applicable.

Explanation

• Biotin Assay Medium (USP) is not a synthetic reagent or building block and does not participate in organic or inorganic transformations. For chemical synthesis applications, refer instead to individual reagents, catalysts, or solvents suited to the target reaction chemistry.

Target Specificity

Not applicable.

Explanation

• Target specificity, epitopes, clones, and isotypes pertain to antibodies and affinity reagents. Biotin Assay Medium (USP) is a culture medium and has no target-binding specificity.