BT57 Reagent - BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC)

Cat. No.: B1523260

GRADE & PURITY BioReagent, Suitable for mass spectrometry (MS), ≥99%(HPLC)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Protein/Antibody Characterization

Übersicht

BT57 is an all-in-one reagent for rapid denaturation, reduction and alkylation. In biopharmaceutical and proteomics research, native proteins with three-dimensional structures undergo denaturation, reduction, alkylation, desalting, and subsequent enzymatic digestion. Traditional denaturants such as urea and guanidine hydrochloride can cause chemical modifications to proteins, such as carbamylation. In addition, conventional alkylating reagents require protection from light. BT57 all-in-one reagent enables one-step addition and rapidly completes protein denaturation, reduction and alkylation. It is suitable for proteomics, biopharmaceutical and single-cell sample research. Physical Form: Lyophilized powder containing 1.5mg BT Surfactant, TCEP, ABC and CAA. Reconstitution: Reconstitute with 52 μL of ultrapure water. pH Range: pH 7–9 for protein denaturation. The reagent degrades and precipitates in solution at pH 2–4. Usage: Add 10 μL of BT57 reagent to 10 μL (50–100 μg) protein sample, mix well. Incubate in a dry bath at 60 °C for 20 min, or at 95 °C for 10 min.

Specifications

Spezifikationen & Reinheit

BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC)

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

BioReagent, Geeignet für die Massenspektrometrie (MS)

Reinheit

≥99%(HPLC)