Camonsertib - ≥99% , CAS No.2417489-10-0

CAS: 2417489-10-0 Cat. No.: C651475 Molecular Weight: 410.47

GRADE & PURITY ≥99%

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Overview

Camonsertib (RP-3500) is an orally active, selective ATR kinase inhibitor (ATRi) with an IC 50 of 1.00 nM in biochemical assays. Camonsertib shows 30-fold selectivity for ATR over mTOR (IC 50 =120 nM) and >2,000-fold selectivity over ATM , DNA-PK , and PI3Kα kinases. Camonsertib has potent antitumor activity

In Vitro

Camonsertib (RP-3500; 1 μM; 1-24 hours) inhibits CHK1(Ser345) phosphorylation from 1 to 3 hours. ?\nCamonsertib inhibits Gemcitabine stimulated ATR phosphorylation of its substrate pCHK1(Ser345) with an IC 50 of 0.33 nM in a LoVo cell-based assay. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: LoVo and CW-2 human colon cancer cell lines Concentration: 1 μM Incubation Time: 1, 2, 4, 6, 8, 16, 24 hours Result: Inhibited CHK1(Ser345) phosphorylation from 1 to 3 hours. Starting at 4 hours, CHK1(Ser345) became re-phosphorylated as DNA-PKcs became activated in treated cells, along with its substrates KAP1 and H2AX.

In Vivo

Camonsertib (RP-3500; 3, 7, 15 mg/kg; Orally; once daily for 18 days) produces dose-dependent tumor growth inhibition with a minimum effective dose (MED) of 7 mg/kg in LoVo xenografts . ?\nCamonsertib (5, 10 mg/kg; Orally; once daily) produces statistically significant tumor growth inhibition in the CW-2 colon xenograft model . ?\nCamonsertib (7 mg/kg; for 7 days) results in 8.1- and 2.7-fold inductions of KAP1 and DNA-PKcs phosphorylation in mice bearing LoVo tumors . ?\nCamonsertib has a more profound anti-tumor effect occurred at higher doses on the 3 days on/4 days off (30 mg/kg) and 5 days on/2 days off (25 mg/kg) schedules compared with consecutive daily administrations (10 mg/kg) at a lower dose for 14 days . ?\nCamonsertib (15mg/kg) combined PARPi Olaparib (80mg/kg; both agents days 1-3 on/4 days off) or sequential (PARPi for 3 days followed by RP-3500 for 3 days then 1 day off) schedules produces greater antiTumor effects compared with sequential administration without affecting tolerability . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female mice (6-8 weeks old) bearing LoVo xenografts Dosage: 3, 7, 15 mg/kg (0.5% methylcellulose/0.02% SDS vehicle) Administration: Orally; once daily for 18 days Result: Produced dose-dependent tumor growth inhibition with a minimum effective dose (MED) of 7 mg/kg. The maximum tolerated dose (MTD) was 10 mg/kg once daily on a continuous dosing schedule.

Form:Solid

IC50& Target:ATR ATM >30 μM (IC 50 ) mTOR 120 nM (IC 50 )

Specifications

Specifications & Purity

≥99%

Biochemical and Physiological Mechanisms

Camonsertib (RP-3500) is an orally active, selective ATR kinase inhibitor (ATRi) with an IC 50 of 1.00 nM in biochemical assays. Camonsertib shows 30-fold selectivity for ATR over mTOR (IC 50 =120 nM) and >2, 000-fold selectivity over ATM , DNA-PK , and PI

Storage

Store at 2-8°C, Protected from light, Desiccated

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥99%

Names and Identifiers

• Canonical Smiles: C[C@@H]1COCCN1C2=NC3=C(C=NN3C4=CC=NN4)C(=C2)C5(C[C@H]6CC[C@@H](C5)O6)O
• Isomeric SMILES: C[C@@H]1COCCN1C2=NC3=C(C=NN3C4=CC=NN4)C(=C2)C5(C[C@H]6CC[C@@H](C5)O6)O
• Molecular Weight: 410.47

Chemical and Physical Properties

• Solubility: DMSO : 50 mg/mL (121.81 mM; Need ultrasonic)
• Molecular Weight: 410.500 g/mol
• XLogP3: 1.600
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 3
• Exact Mass: 410.207 Da
• Monoisotopic Mass: 410.207 Da
• Topological Polar Surface Area: 101.000 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 628.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 3
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1