Casein-FITC conjugate

Cat. No.: C275894

GRADE & PURITY Ex:498nm, Em:517nm

Übersicht

Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.

Specifications

Product Name

Casein-FITC conjugate

Synonyme

FITC-Casein | fluorescein isothiocyanate (FITC)-labeled casein

Note

Em:517nm, Ex:498nm

Spezifikationen & Reinheit

Ex:498nm, Em:517nm

Konjugation

FITC

Anregung (nm)

Blue Laser(488nm)

Emission (nm)

517nm

Ex/Em(nm)

Hinweis

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Molekültyp

Protein

Product Line

Biologische Produkte

Lagerung und Versand

Rekonstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the material to approximately 5 mg/ml by addition of 1mL distilled water. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C, avoid repeated freeze-thaw cycles.

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Stabilität und Lagerung

Product should be stored at 2-8℃ in the dark. After reconstitution, store in aliquots and freeze material at -20℃, avoid freeze/thaw cycle.

Häufig gestellte Fragen

What does Ex:498nm indicate?

Ex:498nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

What does Em:517nm indicate?

Em:517nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.