QUALITÄT & REINHEITCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Rabbit anti Human CCT2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-3 μg/mL
IHC: 5-30 μg/mL
IF/ICC: 2.5 μg/mL
Protein Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Spezifikationen
Product Name
CCT2 Antibody - Primärer Antikörper, specific to CCT2, Rabbit IgG
Synonyme
99D8.1 antibody|CCT 2 antibody|CCT beta antibody|CCT-beta antibody|CCT2 antibody|CCTB antibody|Chaperonin containing t complex polypeptide 1 beta subunit antibody|Chaperonin containing t complex polypeptide 1 subunit 2 antibody|Chaperonin containing TCP1 subunit 2 antibody|Chaperonin containing TCP1 subunit 2 (beta) antibody|CTP:phosphocholine cytidylyltransferase 2 antibody|Epididymis secretory sperm binding protein Li 100n antibody|HEL S 100n antibody|MGC142074 antibody|MGC142076 antibody|MGC94480 antibody|PRO1633 antibody|T complex protein 1 beta subunit antibody|T complex protein 1 subunit beta antibody|T-complex protein 1 subunit beta antibody|TCP 1 beta antibody|TCP-1-beta antibody|CCTB|CCT-beta|TCP-1-beta
Spezifikationen & Reinheit
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Wirtsspezies
Kaninchen
Spezifität
CCT2
Immunogen
Recombinant CCT2 expressed in E.coli (AA 1-488).
Positive Kontrolle
WB: HeLa and MCF7 cell lysates. IHC: Human glioma, kidney, stomach, breast cancer, cerebrum, spleen and Porcine cerebrum tissues. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Antigen affinity purified + Protein A purified
Konzentration
See COA
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
CCT2 Antibody (Ab093602) - Western Blot All lanes: CCT2 Antibody (Ab093602) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 54 kDa Exposure time: 12.6 s
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Porcine Cerebrum Tissue Primary Ab: 20 μg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Cerebrum Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IF/ICC
IF analysis of CCT2 Antibody (Ab093602) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CCT2 Antibody (Ab093602) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
5-30 µg/mL
WB
0.01-3 µg/mL
IF/ICC
2.5 µg/mL
Erweiterte Daten
Zugehörige Ziele (menschlich)
CCT2TbioT-complex protein 1 subunit beta (0 Activities)
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