CD3 Mouse mAb (AF594) - Primärer Antikörper, specific to CD3

Cat. No.: Ab189662

GRADE & PURITY Validated, ExactAb™, 5 μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

AF594

Übersicht

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow Cytometry

Specifications

Product Name

CD3 Mouse mAb (AF594) - Primärer Antikörper, specific to CD3

Synonyme

CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a

Spezifikationen & Reinheit

ExactAb™, Validated, 5 μL/test

Biochemische und physiologische Mechanismen

The CD3 complex mediates signal transduction.

Wirtsspezies

Maus

Spezifität

CD3

Immunogen

Human CD3ε

Positive Kontrolle

Flow Cytometry: Human peripheral blood lymphocytes

Konjugation

AF594

Anregung (nm)

Yellow-Green Laser(594nm)

Emission (nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

5 μL/test

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD3 Mouse mAb (AF594) (Ab189662)

CD3 Mouse mAb (AF594) (Ab189662) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with CD3 Mouse mAb (AF594) (Ab189662) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF594) (Ab190247).

Flow Cytometry: CD3 Mouse mAb (AF594) (Ab189662)

CD3 Mouse mAb (AF594) (Ab189662) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with CD3 Mouse mAb (AF594) (Ab189662) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF594) (Ab190247). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Zugehörige Ziele (menschlich)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Mouse mAb (AF594)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.