CD3 Rat mAb - Primärer Antikörper, specific to CD3, Rat IgG2a

Cat. No.: Ab094429

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Activation, Flow Cytometry, Functional Assay, Stimulation

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

KT3

Conjugation

Unconjugated

Übersicht

Rat anti Mouse CD3 Antibody, Monoclonal (KT3), could be used for Flow, in vitro T cell negative selection, in vitro T cell stimulation/activation, ICC, IF and so on.

Application:
Flow: Use at an assay dependent concentration.

in vitro T cell negative selection: Use at an assay dependent concentration. 

in vitro T cell stimulation/activation: Use at an assay dependent concentration. 

ICC/IF: Use at an assay dependent concentration.

Protein Function
The CD3 complex mediates signal transduction.

Specifications

Product Name

CD3 Rat mAb - Primärer Antikörper, specific to CD3, Rat IgG2a

Synonyme

CD3 | T3e | CD3epsilon | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surfa

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Rat

Spezifität

CD3

Immunogen

CBAT6 mouse thymocytes

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotyp

Rat IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Bilder

Flow Cytometry: CD3 Rat mAb (Ab094429)

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 with CD3 Rat mAb (Ab094429) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD3 Rat mAb (Ab094429)

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 (red) with CD3 Rat mAb (Ab094429) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vitro T cell negative selection:

  Use at an assay dependent concentration;

• in vitro T cell stimulation/activation:

  Use at an assay dependent concentration;

Zugehörige Ziele (menschlich)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.