CD3 Rat mAb (PE) - Primärer Antikörper, specific to CD3

Cat. No.: Ab185407

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2b

Clone Number

17A2

Conjugation

PE

Übersicht

Rat anti Mouse CD3 Antibody, Monoclonal (17A2), could be used for Flow Cytometry.

Specifications

Product Name

CD3 Rat mAb (PE) - Primärer Antikörper, specific to CD3

Synonyme

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

The CD3 complex mediates signal transduction.

Wirtsspezies

Rat

Spezifität

CD3

Immunogen

γδTCR-positive T-T hybridoma D1

Positive Kontrolle

Flow Cytometry: C57BL/6 Mouse splenocytes

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD3 Rat mAb (PE) (Ab185407)

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD3 Rat mAb (PE) (Ab185407)

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD3 Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test; For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

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