CD31 Rat mAb - Primärer Antikörper, specific to CD31, Rat IgG2a

Cat. No.: Ab094526

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

390

Conjugation

Unconjugated

Übersicht

Rat anti Mouse CD31 Antibody, Monoclonal (390), could be used for Flow, ICC/IF, Intravital imaging, in vivo CD31 blocking and so on.
Application
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
Intravital imaging: Use at an assay dependent concentration.
in vivo CD31 blocking: Use at an assay dependent concentration.
Protein Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Specifications

Product Name

CD31 Rat mAb - Primärer Antikörper, specific to CD31, Rat IgG2a

Synonyme

Cd31 | Pecam | adhesion molecule | CD31 antigen | CD31 | CD31/EndoCAM | EndoCAM | FLJ34100 | FLJ58394 | GPIIA' | PECA1 | PECAM1 | PECAM-1 | PECAM-1, CD31/EndoCAM | platelet endothelial cell adhesion molecule | platelet endothelial cell adhesion molecule-1

Spezifikationen & Reinheit

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Rat

Spezifität

CD31

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, PBS Only, Validated

Isotyp

Rat IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Bilder

Flow Cytometry: CD31 Rat mAb (Ab094526)

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 with CD31 Rat mAb (Ab094526) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD31 Rat mAb (Ab094526)

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 (red) with CD31 Rat mAb (Ab094526) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

IF/ICC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

Intravital imaging

Use at an assay dependent concentration.

in vivo CD31 blocking

Use at an assay dependent concentration.

Zugehörige Ziele (menschlich)

PECAM1 Tbio Platelet endothelial cell adhesion molecule (0 Activities)

Discover Target: PECAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD31 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.