CD44 Rat mAb (PE) - Primärer Antikörper, specific to CD44

Cat. No.: Ab190175

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

PE

Übersicht

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.

Specifications

Product Name

CD44 Rat mAb (PE) - Primärer Antikörper, specific to CD44

Synonyme

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Wirtsspezies

Rat

Spezifität

CD44

Immunogen

Dexamethasone-induced myeloid leukemia M1 cells

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD44 Rat mAb (PE) (Ab190175)

CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD44 Rat mAb (PE) (Ab190175)

CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Zugehörige Ziele (menschlich)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD44 Rat mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.